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Revealing the Native Molecular Architecture of the Nuclear Periphery using Cryo-Focused-Ion-Beam Milling, Light Microscopy and Electron Tomography

Published online by Cambridge University Press:  04 August 2017

Elizabeth Villa ,
Reika Watanabe ,
Robert Buschauer ,
Kanika Khanna ,
Vinson Lam ,
Jitin Singla  and
Frank Alber
Elizabeth Villa
Affiliation:
Division of Biological Sciences, University of California San Diego, La Jolla, USA
Reika Watanabe
Affiliation:
Division of Biological Sciences, University of California San Diego, La Jolla, USA
Robert Buschauer
Affiliation:
Division of Biological Sciences, University of California San Diego, La Jolla, USA Gene Center, Ludwig Maximillian University, Munich, Germany
Kanika Khanna
Affiliation:
Division of Biological Sciences, University of California San Diego, La Jolla, USA
Vinson Lam
Affiliation:
Division of Biological Sciences, University of California San Diego, La Jolla, USA
Jitin Singla
Affiliation:
Department of Biological Sciences, University of Southern California, Los Angeles, USA
Frank Alber
Affiliation:
Department of Biological Sciences, University of Southern California, Los Angeles, USA

Abstract

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Abstract
Information
Microscopy and Microanalysis , Volume 23 , Supplement S1: Proceedings of Microscopy & Microanalysis 2017 , July 2017 , pp. 1248 - 1249
Copyright
© Microscopy Society of America 2017 

References

[1] Villa, E, M Schaffer, M, JM Plitzko, JM & Baumeister, W Curr Opin Struct Biol 2013). P. 771–777.Google Scholar
[2] Wagner, RF, Schampers, R, Watanabe, R, Persoon, H, et al., Nat. Protoc. 2017). Under revision.Google Scholar
[3] Mahamid, J, Pfeffer, S, Schaffer, M, Villa, E, Danev, R, et al., Science 2016). p. 969–972.Google Scholar
[4] Chaikeeratisak, V, Nguyen, K, Khanna, K, Brilot, AF, et al., Science 2017). p. 194–197.CrossRefGoogle Scholar
[5] Xu, M, Tocheva, IE, Chang, Y, Jensen, JG & Alber, F arXiv:1512.09347 [q-bio.QM] 2016.Google Scholar
[6] The authors acknowledge funding from a NIH Director’s New Innovator Award (to E.V.).Google Scholar