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Resveratrol Ameliorates the Seminiferous Tubules Damages Induced by Finasteride in Adult Male Rats

Published online by Cambridge University Press:  05 October 2020

Amany Mohamed Shalaby Open the ORCID record for Amany Mohamed Shalaby [Opens in a new window] ,
Mohamed Ali Alabiad  and
Dina Fouad El Shaer
Amany Mohamed Shalaby*
Affiliation:
Histology and Cell Biology Department, Faculty of Medicine, Tanta University, Tanta 31527, Egypt
Mohamed Ali Alabiad
Affiliation:
Pathology Department, Faculty of Medicine, Zagazig University, Zagazig 44519, Egypt College of Medicine, Shaqra University, Shaqra, Kingdom of Saudi Arabia
Dina Fouad El Shaer
Affiliation:
Histology and Cell Biology Department, Faculty of Medicine, Tanta University, Tanta 31527, Egypt
*
*Author for correspondence: Amany Mohamed Shalaby, E-mail: [email protected]
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Abstract

Finasteride is commonly used in the management of alopecia and nodular prostatic hyperplasia. It was reported to have a harmful effect on spermatogenesis with subsequent infertility. Thus, this research was to determine the ameliorative effect of resveratrol against testicular damage caused by finasteride. Forty adult male rats were randomly divided into four main groups: group I acted as the control, group II was administrated resveratrol 20 mg/kg/day, group III was administrated finasteride 5 mg/kg/day, and group IV was administrated finasteride and resveratrol as in the previous groups. Finasteride induced a significant decrement in the testosterone and dihydrotestosterone levels. The level of malondialdehyde significantly increased, while the levels of glutathione peroxidase, superoxide dismutase, and catalase significantly decreased in the finasteride-administrated rats. Variable histopathological alterations in the testes were revealed in the form of irregular seminiferous tubules. Some seminiferous tubules appeared with degenerated germinal epithelium. Others showed detachment of their germinal epithelium. Congested blood vessels and homogeneous acidophilic substance in-between tubules were also detected. A significant decrement in PCNA positive cells and a significant increment in Bax expression were demonstrated. Ultrastructural examination showed Sertoli cells with rarefied cytoplasm. Vacuolated cytoplasm, shrunken nuclei, and dilated perinuclear spaces were also revealed in the spermatogonia, primary spermatocytes, and early spermatids. On the contrary, few changes were noticed in rats received resveratrol concomitant with finasteride. This study indicated that resveratrol exerted a potent ameliorative effect against testicular injury caused by finasteride.

Keywords

5-α reductase inhibitorBaxelectron microscopyoxidative stressPCNAtestis
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 26 , Issue 6 , December 2020 , pp. 1176 - 1186
Copyright
Copyright © Microscopy Society of America 2020

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