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The Response of Maize Protoplasts to High Intensity Illumination in Multi-Photon Fluorescence Microscopy

Published online by Cambridge University Press:  02 July 2020

B. L. Lin ,
F. J. Kao ,
P. C. Cheng  and
P. C. Cheng
B. L. Lin
Affiliation:
Institute of Molecular Biology, Academia Sinica, Taipei, Taiwan, 11529 Rep. of China
F. J. Kao
Affiliation:
department of Physics, National Sun Yat-sen University, Kaohsiung, Taiwan, 80424 Rep. of China
P. C. Cheng
Affiliation:
AMIL, Dept. of Electrical Engineering, State University of new York, Buffalo, NY14260USA
P. C. Cheng
Affiliation:
Williamsville East HS, Williamsville, NY14051USA
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Extract

Multi-photon fluorescence microscopy has been cited for its advantage in increased depth penetration due to low linear absorption coefficient of biological specimen in the near infrared (NIR) range. Using a pulsed laser, it is possible to efficiently excite two-photon fluorescence with a high peak power while keeping the average power low to avoid thermal and photochemical damages to the specimen. Currently, mode-locked Ti-sapphire and Cr-Forsterite lasers that generate sub-picosecond pulses are used as the light source for multi-photon fluorescence microscopy. Because of the need of high peak power for efficiently exciting two-photon fluorescence, the relationship between cell damage and peak power has become an interesting and much debated topic in the application of multi-photon fluorescence microscopy. It is conceivable that at high illumination intensity, non-linear photochemical processes may have impacts on cell physiology and viability in ways much different from low illumination in the linear domain.

Type
Advances in Multi-Photon Imaging
Information
Microscopy and Microanalysis , Volume 6 , Issue S2: Proceedings: Microscopy & Microanalysis 2000, Microscopy Society of America 58th Annual Meeting, Microbeam Analysis Society 34th Annual Meeting, Microscopical Society of Canada/Societe de Microscopie de Canada 27th Annual Meeting, Philadelphia, Pennsylvania August 13-17, 2000 , August 2000 , pp. 806 - 807
Copyright
Copyright © Microscopy Society of America

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References

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Supported by Academia Sinica (BLL), Natl. Sci. Council, Rep. of China [NSC-89-2311-B-001-032 (BLL), NSC-88-2311-B-OO 1-087 (FJK), NSC-88-2311-B-OO 1-087 (PCC)] and Mr. and Mrs. Jin-Mu Huang of Aurum Belle Investment Co., (on behalf of the Ge-An Charity, ).Google Scholar