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A Rapid Method for Viral Particle Detection in Viral-Induced Gastroenteritis: A TEM Study

Published online by Cambridge University Press:  08 August 2003

M. John Hicks ,
James P. Barrish ,
Elizabeth S. Hayes ,
Laurie C. Leer ,
Mary K. Estes  and
W.D. Cubitt
M. John Hicks
Affiliation:
Ultrastructural Pathology Laboratory, Department of Pathology, Texas Children's Hospital and Baylor College of Medicine, Houston, TX
James P. Barrish
Affiliation:
Ultrastructural Pathology Laboratory, Department of Pathology, Texas Children's Hospital and Baylor College of Medicine, Houston, TX
Elizabeth S. Hayes
Affiliation:
Ultrastructural Pathology Laboratory, Department of Pathology, Texas Children's Hospital and Baylor College of Medicine, Houston, TX
Laurie C. Leer
Affiliation:
Ultrastructural Pathology Laboratory, Department of Pathology, Texas Children's Hospital and Baylor College of Medicine, Houston, TX
Mary K. Estes
Affiliation:
Division of Molecular Virology, Baylor College of Medicine, Houston, TX
W.D. Cubitt
Affiliation:
Department of Virology, Institute of Child Health, London, England, U.K.
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Abstract

Infectious gastroenteritis is a common cause of hospitalization in the pediatric population. The most frequent cause of gastroenteritis is viral in origin. The purpose of this study was to compare a rapid modified negative-staining TEM method with the conventional pseudoreplica technique in detection of viral particles in fecal samples from children with viral gastroenteritis. The modified negative-staining method resulted in a significantly higher (2.5 ± 0.5, p = 0.02) viral rating score than that for the conventional pseudoreplica technique (1.7 ± 0.4). In addition, the preparation time for the negative-staining method was approximately one fifth that for the conventional pseudoreplica technique. Rapid diagnosis of viral gastroenteritis may be made by ultrastructural detection of viral particles in fecal samples using the negative staining technique.

Keywords

electron microscopyfecalgastroenteritisvirus
Type
Research Article
Information
Microscopy and Microanalysis , Volume 1 , Issue 5 , October 1995 , pp. 185 - 189
Copyright
© 1995 Microscopy Society of America

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