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Rabbit Endothelial Progenitor Cells Derived From Peripheral Blood and Bone Marrow: An Ultrastructural Comparative Study

Published online by Cambridge University Press:  17 March 2022

Hana Duranova Open the ORCID record for Hana Duranova [Opens in a new window] ,
Veronika Valkova Open the ORCID record for Veronika Valkova [Opens in a new window] ,
Lucia Olexikova Open the ORCID record for Lucia Olexikova [Opens in a new window] ,
Barbora Radochova Open the ORCID record for Barbora Radochova [Opens in a new window] ,
Andrej Balazi Open the ORCID record for Andrej Balazi [Opens in a new window] ,
Peter Chrenek Open the ORCID record for Peter Chrenek [Opens in a new window]  and
Jaromir Vasicek Open the ORCID record for Jaromir Vasicek [Opens in a new window]
Hana Duranova*
Affiliation:
AgroBioTech Research Centre, Slovak University of Agriculture, Tr. A. Hlinku 2, Nitra 94976, Slovak Republic
Veronika Valkova
Affiliation:
AgroBioTech Research Centre, Slovak University of Agriculture, Tr. A. Hlinku 2, Nitra 94976, Slovak Republic
Lucia Olexikova
Affiliation:
NPPC, Research Institute for Animal Production Nitra, Institute of Farm Animal Genetics and Reproduction, Hlohovecká 2, Lužianky 951 41, Slovak Republic
Barbora Radochova
Affiliation:
Laboratory of Biomathematics, Institute of Physiology, The Czech Academy of Sciences, Vídeňská 1083, Prague 4 CZ-14220, Czech Republic
Andrej Balazi
Affiliation:
NPPC, Research Institute for Animal Production Nitra, Institute of Farm Animal Genetics and Reproduction, Hlohovecká 2, Lužianky 951 41, Slovak Republic
Peter Chrenek
Affiliation:
NPPC, Research Institute for Animal Production Nitra, Institute of Farm Animal Genetics and Reproduction, Hlohovecká 2, Lužianky 951 41, Slovak Republic Faculty of Biotechnology and Food Science, Institute of Biotechnology, Slovak University of Agriculture, Tr. A. Hlinku 2, Nitra 94976, Slovak Republic
Jaromir Vasicek
Affiliation:
NPPC, Research Institute for Animal Production Nitra, Institute of Farm Animal Genetics and Reproduction, Hlohovecká 2, Lužianky 951 41, Slovak Republic Faculty of Biotechnology and Food Science, Institute of Biotechnology, Slovak University of Agriculture, Tr. A. Hlinku 2, Nitra 94976, Slovak Republic
*
*Corresponding author: Hana Duranova, E-mail: [email protected]
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Abstract

The present study was designed to compare the ultrastructure of early endothelial progenitor cells (EPCs) derived from rabbit peripheral blood (PB-EPCs) and bone marrow (BM-EPCs). After the cells had been isolated and cultivated up to passage 3, microphotographs obtained from transmission electron microscope were evaluated from qualitative and quantitative (unbiased stereological approaches) points of view. Our results revealed that both cell populations displayed almost identical ultrastructural characteristics represented by abundant cellular organelles dispersed in the cytoplasm. Moreover, the presence of very occasionally occurring mature endothelial-specific Weibel–Palade bodies (WPBs) confirmed their endothelial lineage origin. The more advanced stage of their differentiation was also demonstrated by the relatively low nucleus/cytoplasm (N/C) ratios (0.41 ± 0.19 in PB-EPCs; 0.37 ± 0.25 in BM-EPCs). Between PB-EPCs and BM-EPCs, no differences in proportions of cells occupied by nucleus (28.13 ± 8.97 versus 25.10 ± 11.48%), mitochondria (3.71 ± 1.33 versus 4.23 ± 1.00%), and lipid droplets (0.65 ± 1.01 versus 0.36 ± 0.40%), as well as in estimations of the organelles surface densities were found. The data provide the first quantitative evaluation of the organelles of interest in PB-EPCs and BM-EPCs, and they can serve as a research framework for understanding cellular function.

Keywords

bone marrowendothelial progenitor cellsperipheral bloodstereological approachestransmission electron microscopy
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 28 , Issue 3 , June 2022 , pp. 756 - 766
Copyright
Copyright © The Author(s), 2022. Published by Cambridge University Press on behalf of the Microscopy Society of America

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