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Quantitative Laser Scanning Confocal Autofluorescence Microscopy (Lscam) of Normal and Premalignant Colonic Tissues

Published online by Cambridge University Press:  02 July 2020

Hsing-Wen Wang ,
Joseph Willis ,
Michael V. Sivak  and
Joseph A. Izatt
Hsing-Wen Wang
Affiliation:
Departments of Biomedical Engineering, Case Western Reserve University, Cleveland,
Joseph Willis
Affiliation:
Pathology, and Case Western Reserve University, Cleveland, OH44106
Michael V. Sivak
Affiliation:
Medicine, Case Western Reserve University, Cleveland, OH, 44106
Joseph A. Izatt
Affiliation:
Departments of Biomedical Engineering, Case Western Reserve University, Cleveland, Medicine, Case Western Reserve University, Cleveland, OH, 44106
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Extract

Laser-induced fluorescence (LIF) spectroscopy of tissues has been proposed as an adjunct to endoscopy for in vivo gastrointestinal (GI) diagnosis of premalignant lesions. In previous studies, under ultraviolet excitation (350-370 nm), sources of LIF signal differences in normal, and premalignant colonie tissues have been identified including 1) changes in gross tissue morphology, 2) increased hemoglobin absorption in adenomatous tissues, and 3) increased red fluorescence in dysplastic cells. However, many questions remain regarding the specific origins and biochemical correlates of GI tissue autofluorescence.

We report on a study of quantitative ultraviolet LIF spectral microscopy in human colonic tissues using a laser scanning confocal microscope with argon laser excitation 351-364 nm. Frozen sections (6μm) of normal colon (n=10) and tubular adenoma (n=8) were prepared from fresh surgical resection specimens using a previously published protocol. To identify histological components accurately, LSCAM slides were alcohol fixed and H&E histologically stained following confocal imaging.

Type
Neoplasia: Abnormal Cell Growth Or Death/Apoptosis? Insights From Microscopy
Information
Microscopy and Microanalysis , Volume 3 , Issue S2: Proceedings: Microscopy & Microanalysis '97, Microscopy Society of America 55th Annual Meeting, Microbeam Analysis Society 31st Annual Meeting, Histochemical Society 48th Annual Meeting, Cleveland, Ohio, August 10-14, 1997 , August 1997 , pp. 25 - 26
Copyright
Copyright © Microscopy Society of America 1997

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References

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