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Quantitation of Peroxisome Proliferation using Silver Enhanced Immunogold Labeling on Glycol Methacrylate (GMA) Sections

Published online by Cambridge University Press:  02 July 2020

G.D. Gagne ,
A.H. Illi ,
D. Hickman  and
J.A. Fagerland
G.D. Gagne
Affiliation:
Pharmaceutical Products Division, Abbott Laboratories, Abbott Park, IL60064
A.H. Illi
Affiliation:
Pharmaceutical Products Division, Abbott Laboratories, Abbott Park, IL60064
D. Hickman
Affiliation:
Pharmaceutical Products Division, Abbott Laboratories, Abbott Park, IL60064
J.A. Fagerland
Affiliation:
Pharmaceutical Products Division, Abbott Laboratories, Abbott Park, IL60064
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Extract

Assessment of peroxisome proliferation is important in drug safety studies, since peroxisome proliferation often precedes tumor development in rodents. Proliferation can be measured morphometrically on sections by immunostaining for the peroxisomal enzyme catalase. Previous studies have used tissue embedded in acrylic resins such as LR White, which requires tissue sizes of 1-2 mm. Glycol methacrylate (GMA) is an acrylic resin that preserves antigenicity in much larger pieces of tissue. In this study we applied immunogold-silver staining (IGSS) to GMA sections for the quantitation of peroxisome proliferation in rat livers.

Sprague-Dawley rats were given clofibrate, a known peroxisome proliferator, for 14 days. Livers were removed, fixed in neutral buffered formalin for 4 hours, and embedded in GMA. Sections (3 μm thick) were incubated with antibody to catalase followed by protein A-gold (PAG), and the gold label was amplified using a commercial silver enhancement kit. Tissue sections were counterstained with propidium iodide (PI).

Type
Cytochemistry (Light and Electron Histochemistry)
Information
Microscopy and Microanalysis , Volume 4 , Issue S2: Proceedings: Microscopy & Microanalysis '98, Microscopy Society of America 56th Annual Meeting, Microbeam Analysis Society 32nd Annual Meeting, Atlanta, Georgia July 12-16, 1998 , July 1998 , pp. 1094 - 1095
Copyright
Copyright © Microscopy Society of America

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References

References:

1.Beier, K.. J Histochem Cytochem 40 (1992) 115.CrossRefGoogle Scholar
2.Weibel, E. R.. J Cell Biol 42 (1969)68.CrossRefGoogle Scholar