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Qualitative Evaluation of Scanning Electron Microscopy Methods in a Study of the Resin Cement/Dentine Adhesive Interface

Published online by Cambridge University Press:  05 November 2013

Carolina N. d. B. Pereira ,
Bruno Daleprane ,
Paola F. Barbosa ,
Allyson N. Moreira  and
Cláudia S. de Magalhães
Carolina N. d. B. Pereira
Affiliation:
Department of Restorative Dentistry, School of Dentistry, Federal University of Minas Gerais, Avenida Antônio Carlos, 6627, Pampulha, Belo Horizonte, MG 31270-901, Brazil
Bruno Daleprane
Affiliation:
Department of Restorative Dentistry, School of Dentistry, Federal University of Minas Gerais, Avenida Antônio Carlos, 6627, Pampulha, Belo Horizonte, MG 31270-901, Brazil
Paola F. Barbosa
Affiliation:
Center of Microscopy, UFMG, Avenida Antônio Carlos, 6627, Pampulha, Belo Horizonte, MG 31270-901, Brazil
Allyson N. Moreira
Affiliation:
Department of Restorative Dentistry, School of Dentistry, Federal University of Minas Gerais, Avenida Antônio Carlos, 6627, Pampulha, Belo Horizonte, MG 31270-901, Brazil
Cláudia S. de Magalhães*
Affiliation:
Department of Restorative Dentistry, School of Dentistry, Federal University of Minas Gerais, Avenida Antônio Carlos, 6627, Pampulha, Belo Horizonte, MG 31270-901, Brazil
*
*Corresponding author. E-mail: [email protected]
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Abstract

Sample preparation and imaging techniques for scanning electron microscopy (SEM) of dehydrated dental samples can hinder the structural analyses. This study qualitatively evaluated images obtained with two different protocols of SEM preparation and analysis to assess the dentine adhesive interface. The crown and root dentine of 12 bovine incisors were subjected to cementation with the resin cement RelyX U100 or RelyX ARC/SBMP (n = 6). After storage for 7 days in a moist environment at 37 ± 1°C, the dentine samples were dehydrated in an ascending alcohol series, and three specimens from each group were coated with gold or carbon and examined in a high-vacuum (JEOL JSM—6360LV, 10 kV) or low-vacuum (FEI Quanta 200F, 15–30 kV) microscope. Images were obtained at magnifications between 50 and 2,000×, but with different working distances. The use of high vacuum for carbon and gold coating and SEM visualization led to cracks in the samples. A small number of cracks can be described in the specimens subjected to the low-vacuum technique. The protocol for SEM imaging in low vacuum was considered more appropriate for preservation of the integrity of the evaluated structures.

Keywords

resin cementsmicroscopyelectronscanningdentin-bonding agentsdental bonding
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 20 , Issue 1 , February 2014 , pp. 268 - 275
Copyright
Copyright © Microscopy Society of America 2014 

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References

Abou-Id, L.R., Morgan, L.F., Silva, G.A., Poletto, L.T., Lanza, L.D. & Albuquerque Rde, C. (2012). Ultrastructural evaluation of the hybrid layer after cementation of fiber posts using adhesive systems with different curing modes. Braz Dent J 23(2), 116–121.CrossRefGoogle ScholarPubMed
Adebayo, O.A., Burrow, M.F. & Tyas, M.J. (2010). Resin-dentine interfacial morphology following CPP-ACP treatment. J Dent 38(2), 96–105.CrossRefGoogle ScholarPubMed
Adebayo, O.A., Burrow, M.F., Tyas, M.J. & Palamara, J. (2012). Effect of tooth surface preparation on the bonding of self-etching primer adhesives. Oper Dent 37(2), 137–149.Google Scholar
Aguiar, T.R., Andre, C.B., Arrais, C.A.G., Bedran-Russo, A.K. & Giannini, M. (2012). Micromorphology of resin-dentin interfaces using self-adhesive and conventional resin cements: A confocal laser and scanning electron microscope analysis. Int J Adhes Adhes 38, 69–74.Google Scholar
Al-Assaf, K., Chakmakchi, M., Palaghias, G., Karanika-Kouma, A. & Eliades, G. (2007). Interfacial characteristics of adhesive luting resins and composites with dentine. Dent Mater 23(7), 829–839.Google Scholar
Cantoro, A., Goracci, C., Papacchini, F., Mazzitelli, C., Fadda, G.M. & Ferrari, M. (2008). Effect of pre-cure temperature on the bonding potential of self-etch and self-adhesive resin cements. Dent Mater 24(5), 577–583.Google Scholar
Cehreli, Z.C., Yazici, A.R., Akca, T. & Ozgünaltay, G. (2003). A morphological and micro-tensile bond strength evaluation of a single-bottle adhesive to caries-affected human dentine after four different caries removal techniques. J Dent 31(6), 429–435.Google Scholar
Ferrari, M. & Mannocci, F. (2000). A “one-bottle” adhesive system for bonding a fibre post into a root canal: An SEM evaluation of the post-resin interface. Int Endodontic J 33(4), 397–400.CrossRefGoogle Scholar
Giachetti, L., Bertini, F. & Scaminaci Russo, D. (2004). Investigation into the nature of dentin resin tags: A scanning electron microscopic morphological analysis of demineralized bonded dentin. J Prosthet Dent 92(3), 233–238.Google Scholar
Haragushiku, G.A., Teixeira, C.S., Furuse, A.Y., Sousa, Y.T., De Sousa Neto, M.D. & Silva, R.G. (2012). Analysis of the interface and bond strength of resin-based endodontic cements to root dentin. Microsc Res Tech 75(5), 655–661.Google Scholar
Hirabayashi, S., Yoshida, E. & Hayakawa, T. (2011). SEM analysis of microstructure of adhesive interface between resin cement and dentin treated with self-etching primer. Dent Mater J 30(4), 528–536.Google Scholar
Kalkan, M., Usumez, A., Ozturk, A.N., Belli, S. & Eskitascioglu, G. (2006). Bond strength between root dentin and three glass-fiber post systems. J Prosthet Dent 96(1), 41–46.CrossRefGoogle ScholarPubMed
Margvelashvili, M., Goracci, C., Beloica, M., Papacchini, F. & Ferrari, M. (2010). In vitro evaluation of bonding effectiveness to dentin of all-in-one adhesives. J Dent 38(2), 106–112.CrossRefGoogle ScholarPubMed
Montes, M.A., de Goes, M.F., da Cunha, M.R. & Soares, A.B. (2001). A morphological and tensile bond strength evaluation of an unfilled adhesive with low-viscosity composites and a filled adhesive in one and two coats. J Dent 29(6), 435–441.CrossRefGoogle Scholar
Nakabayashi, N., Kojima, K. & Masuhara, E. (1982). The promotion of adhesion by the infiltration of monomers into tooth substrates. J Biomed Mater Res 16(3), 265–273.Google Scholar
Noirrit, E.E., Gregoire, G. & Cournot, M. (2008). Morphological study of fiber-reinforced post-bonding system-root dentin interface by evaluation of two bonding systems. J Dent 36(3), 204–213.Google Scholar
Perdigão, J., Lambrechts, P., Van Meerbeek, B., Vanherle, G. & Lopes, A.L. (1995). Field emission SEM comparison of four postfixation drying techniques for human dentin. J Biomed Mater Res 29(9), 1111–1120.Google Scholar
Van Meerbeek, B., Inokoshi, S., Braem, M., Lambrechts, P. & Vanherle, G. (1992). Morphological aspects of the resin-dentin interdiffusion zone with different dentin adhesive systems. J Dent Res 71(8), 1530–1540.CrossRefGoogle ScholarPubMed
Vaz, R.R., Hipólito, V.D., D'Alpino, P.H. & Goes, M.F. (2012). Bond strength and interfacial micromorphology of etch-and-rinse and self-adhesive resin cements to dentin. J Prosthodont 21(2), 101–111.CrossRefGoogle ScholarPubMed
Yoshiyama, M., Doi, J., Nishitani, Y., Itota, T., Tay, F.R., Carvalho, R.M. & Pashley, D.H. (2004). Bonding ability of adhesive resins to caries-affected and caries-infected dentin. J Appl Oral Sci 12(3), 171–176.Google Scholar
Yuan, Y., Shimada, Y., Ichinose, S. & Tagami, J. (2007). Effect of dentin depth on hybridization quality using different bonding tactics in vivo. J Dent 35(8), 664–672.Google Scholar