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Protein Interaction and Transport Maps of Live Cell Nuclei Using Fluorescence Correlation Spectroscopy in a Single Plane Illumination Microscope

Published online by Cambridge University Press:  27 August 2014

Jan W. Krieger
Affiliation:
Division Biophysics of Macromolecules, German Cancer Research Center, Im Neuenheimer Feld 580, D-69120 Heidelberg, Germany
Agata Pernus
Affiliation:
Division Biophysics of Macromolecules, German Cancer Research Center, Im Neuenheimer Feld 580, D-69120 Heidelberg, Germany
Peter Brazda
Affiliation:
Department of Biochemistry and Molecular Biology, Immunogenomics Research Group, Research Center for Molecular Medicine, University of Debrecen, Hungary
Jörg Langowski
Affiliation:
Division Biophysics of Macromolecules, German Cancer Research Center, Im Neuenheimer Feld 580, D-69120 Heidelberg, Germany

Abstract

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Type
Abstract
Copyright
Copyright © Microscopy Society of America 2014 

References

(1) Dross, N, Spriet, C, Zwerger, M, Muller, G & Waldeck, W, and Langowski, J Mapping eGFP oligomer mobility in living cell nuclei. PLoS One 4 (4), e5041, 2009.Google Scholar
(2) Singh, AP, Krieger, JW, Buchholz, J, Charbon, E & Langowski, J,andWohland, T The performance of 2D array detectors for light sheet based fluorescence correlation spectroscopy. Opt Express 21 (7), 8652-8668, 2013.Google Scholar
(3) Krieger, JW, Singh, AP, Garbe, CS & Wohland, T, andLangowski, J Dual-Color Fluorescence Cross-Correlation Spectroscopy on a Single Plane Illumination Microscope (SPIM-FCCS). Opt Express 22 (3), 2358-2375, 2014.Google Scholar
(4) Baudendistel, N, Müller, G, Waldeck, W & Angel, P, andLangowski, J Two-Hybrid Fluorescence Cross-Correlation Spectroscopy Detects Protein-Protein Interactions In Vivo. Chemphyschem 6 (5), 984-990, 2005.Google Scholar
(5) Brazda, P, Krieger, J, Daniel, B, Jonas, D, Szekeres, T, Langowski, J, Toth, K & Nagy, L, andVamosi, G Ligand binding shifts highly mobile RXR to chromatin-bound state in a coactivator-dependent manner as revealed by single cell imaging. Mol Cell Biol 2014.Google Scholar