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The Pros and Cons of Apoptosis Assays for Use in the Study of Cells, Tissues, and Organs

Published online by Cambridge University Press:  27 November 2002

Michiko Watanabe ,
Midori Hitomi ,
Kathy van der Wee ,
Florence Rothenberg ,
Steven A. Fisher ,
Robert Zucker ,
Kathy K.H. Svoboda ,
Edie C. Goldsmith ,
Kaisa M. Heiskanen  and
Anna-Liisa Nieminen
Michiko Watanabe
Affiliation:
Department of Pediatrics (UHC), Case Western Reserve University, Cleveland, OH 44106, USA
Midori Hitomi
Affiliation:
Department of Pediatrics (UHC), Case Western Reserve University, Cleveland, OH 44106, USA
Kathy van der Wee
Affiliation:
Department of Medicine, Case Western Reserve University, Cleveland, OH 44106, USA
Florence Rothenberg
Affiliation:
Department of Medicine, Case Western Reserve University, Cleveland, OH 44106, USA
Steven A. Fisher
Affiliation:
Department of Medicine, Case Western Reserve University, Cleveland, OH 44106, USA
Robert Zucker
Affiliation:
Reproductive Toxicology Division, National Health and Environmental Effects Research Laboratory, Office of Research and Development, U.S. Environmental Protection Agency, Research Triangle Park, NC 27711, USA
Kathy K.H. Svoboda
Affiliation:
Department of Biomedical Sciences, Texas A&M Health Science Center, Baylor College of Dentistry, Dallas, TX 75246, USA
Edie C. Goldsmith
Affiliation:
Department of Developmental Biology and Anatomy, University of South Carolina School of Medicine, Columbia, SC 29209, USA
Kaisa M. Heiskanen
Affiliation:
Department of Anatomy, Case Western Reserve University, Cleveland, OH 44106, USA Kaisa M. Heiskanen is now at the Department of Biology, Laboratory of Animal Physiology, University of Turku, Finland
Anna-Liisa Nieminen
Affiliation:
Department of Anatomy, Case Western Reserve University, Cleveland, OH 44106, USA CWRU/University Hospitals Ireland Comprehensive Cancer Center, Cleveland, OH 44106, USA
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Abstract

Programmed cell death or apoptosis occurs in many tissues during normal development and in the normal homeostasis of adult tissues. Apoptosis also plays a significant role in abnormal development and disease. Increased interest in apoptosis and cell death in general has resulted in the development of new techniques and the revival of old ones. Each assay has its advantages and disadvantages that can render it appropriate and useful for one application, but inappropriate or difficult to use in another. Understanding the strengths and limitations of the assays would allow investigators to select the best methods for their needs.

Keywords

cell deathconfocal microscopyimmunohistological techniquesmitochondriaprogrammed cell death
Type
BIOLOGICAL REVIEW PAPER
Information
Microscopy and Microanalysis , Volume 8 , Issue 5 , October 2002 , pp. 375 - 391
Copyright
2002 Microscopy Society of America

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