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Preliminary Identification of β-Carotene in the Vitreous Asteroid Bodies by Micro-Raman Spectroscopy and HPLC Analysis

Published online by Cambridge University Press:  01 March 2007

Shan-Yang Lin ,
Ko-Hua Chen ,
Wen-Ting Cheng ,
Chi-Tien Ho  and
Shun-Li Wang
Shan-Yang Lin
Affiliation:
Department of Medical Research & Education, Taipei Veterans General Hospital, Taipei, Taiwan, Republic of China
Ko-Hua Chen
Affiliation:
Department of Ophthalmology, Taipei Veterans General Hospital and National Yang-Ming University, Taipei, Taiwan, Republic of China Medical Engineering Section, National Health Research Institute, Taipei, Taiwan, Republic of China
Wen-Ting Cheng
Affiliation:
Department of Medical Research & Education, Taipei Veterans General Hospital, Taipei, Taiwan, Republic of China
Chi-Tien Ho
Affiliation:
Department of Medical Research & Education, Taipei Veterans General Hospital, Taipei, Taiwan, Republic of China
Shun-Li Wang
Affiliation:
Department of Applied Chemistry, National ChiaYi University, ChiaYi, Taiwan, Republic of China
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Abstract

β-carotene was first identified from the vitreous asteroid bodies (ABs) excised from one patient with asteroid hyalosis (AH) by confocal Raman microspectroscopy and was also verified by high performance liquid chromatography (HPLC). Two patients had been diagnosed with AH and intervened by surgical vitrectomy due to blurred vision. The morphology and components of both AB specimens were observed by optical microscopy and determined by using confocal Raman microspectroscopy and HPLC analysis, respectively. Surprisingly, two unique peaks at 1528 and 1157 cm−1 were found in the Raman spectrum for the AB specimen of patient 1 alone, which were in close agreement with that of the Raman peaks at 1525 and 1158 cm−1 for β-carotene and/or lutein. However, HPLC analytical data clearly indicated that the retention time for the extracted sample from the AB specimen of patient 1 was observed at 13.685 min and just identical to that of β-carotene (13.759 min) rather than lutein (2.978 min). In addition, the lack of any peak in the HPLC profile for the AB specimen of patient 2 also confirmed the absence of Raman peaks at 1525 and 1158 cm−1. Thus this preliminary study strongly suggests that β-carotene as a unique component of ABs was specifically detected from the AB specimen of one AH patient by using confocal Raman microspectroscopy and HPLC analysis.

Keywords

asteroid hyalosisasteroid bodiesRamanHPLCβ-carotenelutein
Type
BIOLOGICAL APPLICATIONS
Information
Microscopy and Microanalysis , Volume 13 , Issue 2 , April 2007 , pp. 128 - 132
Copyright
© 2007 Microscopy Society of America

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