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Precise Microanalysis of Biological Remains Using a Process of Laser Ablation

Published online by Cambridge University Press:  02 July 2020

Edward F. Plinski  and
Jerzy S. Witkowski
Edward F. Plinski
Affiliation:
Wroclaw University of Technology, Wybrzeze Wyspianskiego 27, 50-370, Wroclaw, Poland e-mail: [email protected]
Jerzy S. Witkowski
Affiliation:
Wroclaw University of Technology, Wybrzeze Wyspianskiego 27, 50-370, Wroclaw, Poland e-mail: [email protected]
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Abstract

A precise analysis of the biological remains is presented in the paper. A single-channel waveguide carbon dioxide laser is used in a process of ablation ‘ for the exposing of biological remains of mineralised organisms found in a sea slime used as a component in some technological process.

Many important applications of the laser base on the process of ablation. These include also so precise biomedical uses like structural studies of biomolecules. Among of the wide range of the laser devices the waveguide carbon dioxide lasers can be used in a precise biological investigations. A single-channel waveguide CO2 laser emits very easy the optical wave formed in a single, so called quasi-Gaussian, mode2. The distribution of the intensity of the laser output radiation in a basic Gaussian mode is shown in Figure 1. A high quality laser output beam in a basic Gaussian mode can be easy focused permitting a precise ablation of the investigated material in a very small area of 100 μm and less.

Type
Microscopy in the Real World: Natural Materials
Information
Microscopy and Microanalysis , Volume 7 , Issue S2: Proceedings: Microscopy & Microanalysis 2001, Microscopy Society of America 59th Annual Meeting, Microbeam Analysis Society 35th Annual Meeting, Long Beach, California August 5-9, 2001 , August 2001 , pp. 540 - 541
Copyright
Copyright © Microscopy Society of America 2001

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References

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2.) Plinski, E. F., Baczyk, K., Abramski, K. M., Witkowski, J. S., Nowicki, R., “60 W single EH11 mode waveguide CO2 laser”, Laser Technology V: Physics and research and Development Trends, Swinoujscie, 1996, Proc.SPIE, 3186(1997)275–278.Google Scholar

3.) Plinski, E. F., Gawlinski, M., “Laser marking in sealing rings”, Institute of Telecommunications and Acoustics, and Institute of Heat Technique and Mechanics of Liquids, Wroclaw University of Technology, Wroclaw, 1996, unpublicised paper.Google Scholar

4.) Plinski, E. F., Witkowski, J. S., Abramski, K. M., “Difractive mechanism for laser marker”, Opt.Las.Techn., 32(2000)33–37.CrossRefGoogle Scholar

5.) Plinski, E. F., Majewski, B. W., Bednarczyk, A. S., Abramski, K. M., “Pulse mode operation of RF excited CO2 waveguide laser”, XII International Symposium on Gas Flow and Chemical Lasers and High-Power Laser Conference, 31 Aug. - 5 Sept. 1998, Sankt Petersburg Proc.SPIE, 3574(1998) 500–503.Google Scholar

6.) This research was partly supported by the KBN Grant No. 8 T11B 021 18.Google Scholar