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The Precise Alignment and Auto-fusion of Correlative Cryo-SXT and Cryo-FM.

Published online by Cambridge University Press:  10 August 2018

Haobo Bai ,
Yong Guan ,
Liang Chen ,
Gang Liu  and
Yangchao Tian
Haobo Bai
Affiliation:
National Synchrotron Radiation Laboratory, University of Science and Technology of China, Hefei, Anhui, People's Republic of China
Yong Guan
Affiliation:
National Synchrotron Radiation Laboratory, University of Science and Technology of China, Hefei, Anhui, People's Republic of China
Liang Chen
Affiliation:
National Synchrotron Radiation Laboratory, University of Science and Technology of China, Hefei, Anhui, People's Republic of China
Gang Liu
Affiliation:
National Synchrotron Radiation Laboratory, University of Science and Technology of China, Hefei, Anhui, People's Republic of China
Yangchao Tian*
Affiliation:
National Synchrotron Radiation Laboratory, University of Science and Technology of China, Hefei, Anhui, People's Republic of China
*
*Yangchao Tian, [email protected]

Abstract:

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Cell's sub-cellular architecture and function can be revealed by Cryo soft X-ray tomography (Cryo-SXT) and Cryo-fluorescence microscopy (Cryo-FM) respectively. To understand the connection between cells' structure and function, correlative Cryo-SXT and Cryo-FM has been applied. Here we introduce a semiautomatic precise alignment and fusion technology of Cryo-SXT and Cryo-fluorescence microscopy.

Type
Abstract
Information
Microscopy and Microanalysis , Volume 24 , Supplement S2: Proceedings of the 14th International Conference on X-ray Microscopy (XRM2018) , August 2018 , pp. 140 - 141
Copyright
© Microscopy Society of America 2018 

References

References:

[1] Dubochet, J Blanc, NS Micron 32 2001 91–99.Google Scholar
[2] Chichon, FJ, et al, J. Struct. Biol. 177 2012 202–211.Google Scholar
[3] Guo, L, et al, Proceedings of the SPIE 2017) 102551Q.Google Scholar
[4] Smith, EA, et al, J. Struct. Biol. 184 2013 12–20.Google Scholar
[5] Cheezum, MK, et al, Biophys. J. 81 2001 2378–2388.Google Scholar
[6] Larson, DR, et al, Biophys. J. 82 2002 2775–2783.Google Scholar
[7] Schellenberger, P, et al, Ultramicroscopy 143 2014 41–51.Google Scholar