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The Potential for Correlative and Multiple Labeling With Colloidal Particles of Differing Shapes

Published online by Cambridge University Press:  02 July 2020

R.M. Albrecht  and
D.A. Meyer
R.M. Albrecht
Affiliation:
Department of Animal Health and Biomedical Sciences, University of Wisconsin, Madison, WI53706
D.A. Meyer
Affiliation:
Department of Animal Health and Biomedical Sciences, University of Wisconsin, Madison, WI53706
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Extract

Labeling specific tissues, cells, cell components, or individual molecular species is often critical to correlative microscopic studies. In the case of larger structures such as tissues or cells it is often possible to use fluoresecent labels or cytologic/cytochemical based stains which are observable via photon based imaging systems. Specific locations on cells and tissues identified by the labeling or staining procedure can be delineated and then can be viewed via electron or force based instrumentation. This is provided preparative methodologies can be designed which: 1. are compatible with observation of the particular sample by the various desired imaging modes and 2. do not compromise structure viewed at the higher levels of resolution provided by electron and force based imaging technologies. This approach is particularly valuable where simultaneous multiple labeling is required since color differences permit discrimination of differing fluorescent and/or chromatic stains.

Type
Labeling for Microscopy and Correlative Microscopy
Information
Microscopy and Microanalysis , Volume 6 , Issue S2: Proceedings: Microscopy & Microanalysis 2000, Microscopy Society of America 58th Annual Meeting, Microbeam Analysis Society 34th Annual Meeting, Microscopical Society of Canada/Societe de Microscopie de Canada 27th Annual Meeting, Philadelphia, Pennsylvania August 13-17, 2000 , August 2000 , pp. 318 - 319
Copyright
Copyright © Microscopy Society of America

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References

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