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Possibilities And Examples For Remote Microscopy Including Digital Image Acquisition, Transfer, and Archiving

Published online by Cambridge University Press:  02 July 2020

I. Daberkow
Affiliation:
Soft Imaging System, Hammer Strasse 89, D-48153, Muenster, Germany
M. Schierjott
Affiliation:
Soft Imaging System, Hammer Strasse 89, D-48153, Muenster, Germany
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Extract

Recent developments promise the possibility to externally control every aspect of microscopes through a computer interface. In combination with high-resolution cameras and feedback to the microscope, this can be leveraged to create highly automatic routines, e.g., to remotely correct astigmatism. Together with the development of fast computer networks this creates a new branch of microscopy, the so-called “telemicroscopy”. The goal of telemicroscopy is the control of a microscope over a large distance including the transfer of images with an acceptable repetition rate. A big advantage for electron microscopy in particular is the possibility of having access to expensive and well-equipped microscopes. In the field of light microscopy the branch “telemedicine” was created, meaning the “virtual” presence of a colleague or specialist for discussion or diagnosis.

Using transmission electron microscopy as an example, the history and special requirements for automation and telemicroscopy will be discussed. In the late 80's the first TEM with a remote control was revealed. Shortly thereafter, first automatic functions for defocus control and astigmatism correction were developed using a video camera as electronic image converter.

Type
Advances in Remote Microscopy, Instrument Automation and Data Storage
Copyright
Copyright © Microscopy Society of America

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