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Placing TEM Negative Images into the Digital Domain

Published online by Cambridge University Press:  02 July 2020

J.A. Mascorro ,
T. Frasch  and
R.D. Yates
J.A. Mascorro
Affiliation:
Department of Structural and Cellular Biology, , , ,
T. Frasch
Affiliation:
Office of the Vice-Dean Tulane University Health Sciences Center, Tulane University School of Medicine, New Orleans, LA, 70112
R.D. Yates
Affiliation:
Department of Structural and Cellular Biology, , , ,
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Abstract

Several thousand images of various biological tissues have been produced by these investigators (*) over careers spanning many, many years. The majority of the images are preserved on Kodak Electron Image Film #4463, Kodak Electron Microscopy Film #4489, Kodak Electron Image Film #SO 163, or Dupont Ortho S Litho (COS-7) film. These 7 mil ESTAR thick base films were the standards utilized by the biological microscopist. The emulsion was covered with a thin layer of hardened gelatin in order to minimize abrasion. After exposure to the electron beam in the 60-80 kV range, the negatives were developed according to routine photographic procedures employing Kodak D-19 developer, hardened in Kodak fixer, washed for the appropriate time, and stored in glassine envelopes in a dust-free environment. The finished negative with the captured image proves to be long-lasting and will produce an image, today, displaying the very same excellent qualities as did original prints produced years ago.

Type
Ask the Experts: Addressing Issues in Digital Imaging for the Microscopist (Organized by J. Mascorro, R. Anderson and D. Sherman)
Information
Microscopy and Microanalysis , Volume 7 , Issue S2: Proceedings: Microscopy & Microanalysis 2001, Microscopy Society of America 59th Annual Meeting, Microbeam Analysis Society 35th Annual Meeting, Long Beach, California August 5-9, 2001 , August 2001 , pp. 838 - 839
Copyright
Copyright © Microscopy Society of America 2001

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References

1Rose, C., Adobe Photoshop 5.5, Indianapolis, Sams Publishing CompanyGoogle Scholar
2.Appreciation extended to: Mr. Mike Britt, Tulane University Health Sciences Center, Department of Photography and Digital Microscopy.Google Scholar