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Phytofluors: Phytochrome-Based Orange Fluorescent Protein Probes

Published online by Cambridge University Press:  02 July 2020

J. Clark Lagarias ,
Beronda L. Montgomery ,
John T. Murphy  and
Shu-Hsing Wu
J. Clark Lagarias
Affiliation:
Section of Molecular and Cellular Biology, University of California, Davis, CA95616
Beronda L. Montgomery
Affiliation:
Section of Molecular and Cellular Biology, University of California, Davis, CA95616
John T. Murphy
Affiliation:
Section of Molecular and Cellular Biology, University of California, Davis, CA95616
Shu-Hsing Wu
Affiliation:
Section of Molecular and Cellular Biology, University of California, Davis, CA95616
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Plants sense the light environment using pigment-protein complexes that discriminate light color, intensity, duration and direction. The most well-studied of these photoreceptors are the phytochromes, a family of soluble biliproteins found in plants, algae and cyanobacteria. Owing to the linear tetrapyrrole pigment phytochromobilin (PΦB) or phycocyanobilin (PCB) that is covalently linked to a large polypeptide via a thioether linkage, phytochromes perceive differences in the quality and quantity of light via their ability to photointerconvert between red (λmax660 nm) and far-red (λmax730 nm) light absorbing forms. Due to an efficient Z,E photoisomerization of the double bond between the C and D-ring pyrroles, phytochromes are nonfluorescent proteins with fluorescent quantum yields less than 10“3 at room temperature (Figure 1).

Phytochrome genes have been cloned from a wide variety of photosynthetic organisms.

Type
Novel Approaches to Microscopy Of Living Cells
Information
Microscopy and Microanalysis , Volume 5 , Issue S2: Proceedings: Microscopy & Microanalysis '99, Microscopy Society of America 57th Annual Meeting, Microbeam Analysis Society 33rd Annual Meeting, Portland, Oregon August 1-5, 1999 , August 1999 , pp. 1050 - 1051
Copyright
Copyright © Microscopy Society of America

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References

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4. This research was supported by grants from NSF (MCB 9604511) and USDA (AMD 9503140).Google Scholar