Hostname: page-component-586b7cd67f-vdxz6 Total loading time: 0 Render date: 2024-11-28T06:06:26.018Z Has data issue: false hasContentIssue false
  • English
  • Français

Phagocytosis of PLGA Microparticles in Rat Peritoneal Exudate Cells: A Time-Dependent Study

Published online by Cambridge University Press:  30 May 2006

Anderson de Jesus Gomes ,
Claure Nain Lunardi ,
Flávio Henrique Caetano ,
Laurelúcia Orive Lunardi  and
Antonio Eduardo da Hora Machado
Anderson de Jesus Gomes
Affiliation:
Laboratório de Fotoquímica, Instituto de Química, Universidade Federal de Uberlândia, P.O. Box 593, CEP 38400-089 Uberlândia, MG, Brazil
Claure Nain Lunardi
Affiliation:
Laboratório de Farmacologia, Faculdade de Ciências Farmacêuticas de Ribeirão Preto—USP, Av. Café s/n CEP 14040-903 Ribeirão Preto, SP, Brazil
Flávio Henrique Caetano
Affiliation:
Instituto de Biociências, Universidade Estadual Júlio de Mesquita Filho, Av: 24 N1515 CEP 13506-900 Rio Claro, SP, Brazil
Laurelúcia Orive Lunardi
Affiliation:
Instituto de Biociências, Universidade Estadual Júlio de Mesquita Filho, Av: 24 N1515 CEP 13506-900 Rio Claro, SP, Brazil
Antonio Eduardo da Hora Machado
Affiliation:
Laboratório de Fotoquímica, Instituto de Química, Universidade Federal de Uberlândia, P.O. Box 593, CEP 38400-089 Uberlândia, MG, Brazil
Get access

Abstract

With the purpose of enhancing the efficacy of microparticle-encapsulated therapeutic agents, in this study we evaluated the phagocytic ability of rat peritoneal exudate cells and the preferential location of poly(d,l-lactide-co-glycolic acid) (PLGA) microparticles inside these cells. The microparticles used were produced by a solvent evaporation method and were characterized by dynamic light scattering (DLS), transmission electron microscopy (TEM), and scanning electron microscopy (SEM). Size distribution analysis using DLS and SEM showed that the particles were spherical, with diameters falling between 0.5 and 1.5 μm. Results from cell adhesion by SEM assay, indicated that the PLGA microparticles are not toxic to cells and do not cause any distinct damage to them as confirmed by the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assay. Among the large variety of cell populations found in the peritoneal exudates (neutrophils, eosinophils, monocytes, and macrophages), TEM showed that only the latter phagocytosed PLGA microparticles, in a time-dependent manner. The results obtained indicate that the microparticles studied show merits as possible carriers of drugs for intracellular delivery.

Keywords

microparticlesdrug deliveryperitoneal exudate cellsmacrophageTEMSEM
Type
BIOLOGICAL APPLICATIONS
Information
Microscopy and Microanalysis , Volume 12 , Issue 5 , October 2006 , pp. 399 - 405
Copyright
© 2006 Microscopy Society of America

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Ahsan, F., Rivas, I.P., Khan, M.A., & Suárez, A.I.T. (2002). Targeting to macrophage: Role of physicochemical properties of particulate carriers—lipossomes and microsperes—on the phagocytosis by macrophages. J Contr Release 79, 29–40.Google Scholar
Artursson, P., Arro, E., Edman, P., Ericsson, J.L.E., & Sjöholm, I. (1987). Biodegradable microsphere V: Stimulation of macrophages with microparticles made of various polysaccharides. J Pharm Sci 76, 127–133.Google Scholar
Castelli, F., Pitarresi, G., & Giammona, G. (2000). Influence of different parameters on drug release from hydrogel systems to a biomembrane model. Evaluation by differential scanning calorimetry technique. Biomaterials 21, 821–833.Google Scholar
Dunne, M., Corrigan, O.I., & Ramtoola, Z. (2000). Influence of particle size and dissolution conditions on the degradation properties of polylactide-co-glycolide particles. Biomaterials 21, 1659–1668.Google Scholar
Esposito, E., Sebben, S., Cortesi, R., Menegatti, E., & Nastruzzi, C. (1999). Preparation and characterization of cationic microspheres for gene delivery. Int J Pharm 189, 29–41.Google Scholar
Fu, F., Li, X., & Wu, C. (2002). Encapsulation of phthalocyanines in biodegradable poly(sebacic anhydride) nanoparticles. Langmuir 18, 3843–3847.Google Scholar
Gomes, A.J., Lunardi, L.O., Marchetti, J.M., Lunardi, C.N., & Tedesco, A.C. (2005). Photobiological and ultrastructural studies of nanoparticle of poly(lactic-co-glycolic acid)-containing bacteriochlorophyll-a as a photosensitizer useful for PDT treatment. Drug Deliv 12, 1–6.Google Scholar
Hayat, M.A. (1981). Fixation for Electron Microscopy. London: Academic Press Inc.
Jain, R.A. (2000). The manufacturing techniques of various drug loaded biodegradable poly(lactide-co-glycolide) (PLGA) devices. Biomaterials 21, 2475–2490.Google Scholar
Jeon, H.-J., Jeong, Y.-I., Jang, M.-K., Park, Y.-H., & Nah, J.-W. (2000). Effect of solvent on the preparation of surfactant-free poly(DL-lactide-co-glycolide) nanoparticles and norfloxacin release characteristics. Int J Pharm 207, 99–108.Google Scholar
Konan, Y.N., Chevallier, J., Gurny, R., & Allémann, E. (2003). Encapsulation of p-THPP into nanoparticles: Cellular uptake, subcellular localization and effect of serum on the photodynamic activity. Photochem Photobiol 77, 638–644.Google Scholar
Kreuter, J. (2004). Influence of the surface properties on nanoparticle-mediated transport of drugs to the brain. J Nanosci Nanotechnol 4, 484–488.Google Scholar
Kumar, M.N.V.R., Bakowsky, U., & Lehr, C.M. (2004). Preparation and characterization of cationic PLGA nanospheres as DNA carriers. Biomaterials 25, 1771–1777.Google Scholar
Lacasse, F.X., Fillion, M.C., Phillips, N.C., Escher, E., McMullen, J.N., & Hildgen, P. (1998). Influence of surface properties at biodegradable microsphere surfaces: Effects on plasma protein adsorption and phagocytosis. Pharm Res 15, 312–317.Google Scholar
Lamprecht, A., Schäfer, U., & Lehr, C.-M. (2001). Size-dependent bioadhesion of micro- and nanoparticulate carriers to the inflamed colonic mucosa. Pharm Res 18, 788–793.Google Scholar
Lemoine, D., Wauters, F., Bouchend'homme, S., & Preat, V. (1998). Preparation and characterization of alginate microspheres containing a model antigen. Int J Pharm 176, 9–19.Google Scholar
Moghimi, S.M., Hunter, A.C., & Murray, J.C. (2001). Long-circulating and target specific microparticles: Theory to practice. Pharmacol Rev 53, 283–318.Google Scholar
Murakami, H., Kobayashi, M., Kamada, M., Takeuchi, H., & Kawashima, Y. (1999). Preparation of poly(DL-lactide-co-glycolide) nanoparticles by modified spontaneous emulsification solvent diffusion method. Int J Pharm 187, 143–149.Google Scholar
Okada, H. & Toguchi, H. (1995). Biodegradable microspheres in drug delivery. Crit Rev Ther Drug Carrier Syst 12, 1–99.Google Scholar
Panyam, J. & Labhasetwar, V. (2003). Biodegradable nanoparticles for drug and gene delivery to cells and tissue. Adv Drug Deliv Rev 55, 329–347.Google Scholar
Panyam, J., Sahoo, S.K., Prabha, S., Bargar, T., & Labhasetwar, V. (2003). Fluorescence and electron microscopy probes for cellular and tissue uptake of poly(d,l-lactide-co-glycolide) nanoparticles. Int J Pharm 262, 1–11.Google Scholar
Ruan, G. & Feng, S.-S. (2003). Preparation and characterization of poly(lactic acid)-poly(ethyleneglycol)-poly(lactic acid) (PLA-PEG-PLA) microspheres for controlled release of paclitaxel. Biomaterials 24, 5037–5044.Google Scholar
Sahoo, S.K., Panyam, J., Prabha, S., & Labhasetwar, V. (2002). Residual polyvinyl alcohol associated with poly (d,l-lactide-co-gly-colide) nanoparticles affects their physical properties and cellular uptake. J Control Release 82, 105–114.Google Scholar
Tabata, Y. & Ikada, Y. (1988). Effect on the size and surface charge of polymer microspheres on their phagocytosis by macrophage. Biomaterials 9, 356–362.Google Scholar
Tunçay, M., Çalis, S., Kas, H.S., Ercan, M.T., Peksoy, I., & Hincal, A.A. (2000). Diclofenac sodium incorporated PLGA (50:50) microspheres: Formulation considerations and in vitro:in vivo evaluation. Int J Pharm 195, 179–188.Google Scholar