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Oxidative Stress and Lipid Peroxides in the Retina of the BBZ/WOR Diabetic Rat: An Ultrastructural Cytochemical Study

Published online by Cambridge University Press:  02 July 2020

E. Ann Ellis ,
Dennis L. Guberski ,
Maria B. Grant  and
Donald Armstrong
E. Ann Ellis
Affiliation:
Department of Medicine, College of Medicine and Department of Small Animal Clinical Science, College of Veterinary Medicine, University of Florida, Gainesville, FL32610 Department of Small Animal Clinical Science, College of Veterinary Medicine, University of Florida, Gainesville, FL32610
Dennis L. Guberski
Affiliation:
Biomedical Research Models Inc., Rutland, MA
Maria B. Grant
Affiliation:
Department of Medicine, College of Medicine and Department of Small Animal Clinical Science, College of Veterinary Medicine, University of Florida, Gainesville, FL32610
Donald Armstrong
Affiliation:
Department of Small Animal Clinical Science, College of Veterinary Medicine, University of Florida, Gainesville, FL32610
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Extract

Biochemical studies have documented a role for oxidative stress in complications of diabetes. Our previous cytochemical and immunocytochemical studies demonstrated increased levels of NADH oxidase in retinal endothelial cells and pericytes of the BBZ/Wor diabetic rat which coincided with disruption of the blood retinal barrier. Lipid peroxidation and damage to outer segments was demonstrated in retinas of a streptozotocin-induced rat model of diabetes. Xanthine oxidase has been proposed as a source of superoxide and hydrogen peroxide (H2O2) in oxidative stress mediated complications of diabetes; however, there are no studies which confirm the role and cellular sites of xanthine oxidase in complications of diabetes. In addition, cellular sites of lipid peroxides have not been documented in diabetic retinopathy.

Xanthine oxidase was localized in retinas of BBZ/Wor diabetic rat by cerium based histochemistry using hypoxanthine and NADH as substrates.

Type
Labeling for Microscopy and Correlative Microscopy
Information
Microscopy and Microanalysis , Volume 6 , Issue S2: Proceedings: Microscopy & Microanalysis 2000, Microscopy Society of America 58th Annual Meeting, Microbeam Analysis Society 34th Annual Meeting, Microscopical Society of Canada/Societe de Microscopie de Canada 27th Annual Meeting, Philadelphia, Pennsylvania August 13-17, 2000 , August 2000 , pp. 336 - 337
Copyright
Copyright © Microscopy Society of America

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References

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5. This research was supported by NIH Grant EY07739 and EY12601. the American Heart Association and the Department of Health and Rehabilitative Services of the State of Florida for the University of Florida Diabetes Research, Education and Treatment Center.Google Scholar