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Optical Non-Linearities in Infrared Mircospectroscopy: A Preliminary Study into the Effects of Sample Size and Shape on Photometric Accuracy

Published online by Cambridge University Press:  02 July 2020

S.A. Stewart  and
A.J. Sommer
S.A. Stewart
Affiliation:
Molecular Microspectroscopy Laboratory, Dept. of Chemistry, Miami University, Oxford, OH45056
A.J. Sommer
Affiliation:
Molecular Microspectroscopy Laboratory, Dept. of Chemistry, Miami University, Oxford, OH45056
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Extract

Several years ago this laboratory reported on the effects of stray light in an infrared microscope and its consequences on the spatial resolution achievable in a typical line scan or mapping experiment of a polymer film. In this study it was demonstrated that unless the size of the sampled area was greater than twice the wavelength of light employed for the analysis, a significant amount of stray light from near neighbors was contained in the sampled areas. Further, this generalization is only valid for sample sizes above the diffraction limit. The results of this study are summarized in Table. The ratio of the sampled area to that of the aperture size (corresponding to expected sample size) can be thought of as a degradation factor in spatial resolution. In this earlier study the sample geometry was chosen to minimize stray light effects arising from the sample size and shape.

Type
Optical Microanalysis
Information
Microscopy and Microanalysis , Volume 3 , Issue S2: Proceedings: Microscopy & Microanalysis '97, Microscopy Society of America 55th Annual Meeting, Microbeam Analysis Society 31st Annual Meeting, Histochemical Society 48th Annual Meeting, Cleveland, Ohio, August 10-14, 1997 , August 1997 , pp. 837 - 838
Copyright
Copyright © Microscopy Society of America 1997

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References

1.Sommer, A.J.and Katon, J.E., Applied Spectroscopy, 45 (1991), 1633.10.1366/0003702914335229CrossRefGoogle Scholar
2.Reffner, J.andMartoglio, P.A., Practical Guide to Infrared Microspectroscopy, Marcel Dekker (1995).Google Scholar