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Optical Coherence Microscopy: A New Technique for High-Resolution, Non-Invasive Imaging in Bulk Biological Tissues

Published online by Cambridge University Press:  02 July 2020

Joseph A. Izatt ,
Manish Kulkarni ,
Hsing-Wen Wang  and
Michael V. Sivak
Joseph A. Izatt
Affiliation:
Department of Biomedical Engineering, Case Western Reserve University, Cleveland, OH44106 Department of Medicine, Case Western Reserve University, Cleveland, OH44106
Manish Kulkarni
Affiliation:
Department of Biomedical Engineering, Case Western Reserve University, Cleveland, OH44106 Department of Medicine, Case Western Reserve University, Cleveland, OH44106
Hsing-Wen Wang
Affiliation:
Department of Biomedical Engineering, Case Western Reserve University, Cleveland, OH44106 Department of Medicine, Case Western Reserve University, Cleveland, OH44106
Michael V. Sivak
Affiliation:
Department of Biomedical Engineering, Case Western Reserve University, Cleveland, OH44106 Department of Medicine, Case Western Reserve University, Cleveland, OH44106
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Extract

Optical coherence microscopy (OCM) is a novel technique complementary to optical coherence tomography (OCT) which combines low-coherence interferometry with confocal microscopy to achieve micron-scale resolution imaging in highly scattering media. OCM may be implemented using a single-mode fiber-optic low-coherence interferometer (See Fig. 1). A high numerical aperture objective is used to focus sample-arm light into the specimen, and the reference arm length of the interferometer is adjusted to match the sample arm focal plane optical depth. The sample arm of the interferometer comprises a scanning confocal microscope, in which either the sample or the probe beam is laterally scanned in a raster pattern, and the optical fiber acts as a single-mode confocal aperture for combined light illumination and collection. The reference arm length of the interferometer establishes the depth position of an interferometric “coherence gate” in the sample, from which backscattered light is preferentially collected. Initial studies of OCM in scattering phantoms have demonstrated that this technique provides increased optical sectioning depth compared to confocal microscopy alone.

Type
Light Microscopy: Recent Advances
Information
Microscopy and Microanalysis , Volume 3 , Issue S2: Proceedings: Microscopy & Microanalysis '97, Microscopy Society of America 55th Annual Meeting, Microbeam Analysis Society 31st Annual Meeting, Histochemical Society 48th Annual Meeting, Cleveland, Ohio, August 10-14, 1997 , August 1997 , pp. 795 - 796
Copyright
Copyright © Microscopy Society of America 1997

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References

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This research was supported by the National Science Foundation (BES-9624617).Google Scholar