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Ontogenesis of Resin Ducts and Secretory Process in Protium spruceanum (Burseraceae) Stems

Published online by Cambridge University Press:  22 March 2022

Wanessa Stéfanne de Jesus Silva Bruzinga ,
Leonardo Monteiro Ribeiro ,
Yule Roberta Ferreira Nunes ,
Lúcia Pinheiro Santos Pimenta ,
Mariana Araujo Thibau de Almeida ,
Martha Grasielle Alves Mendes  and
Maria Olívia Mercadante-Simões Open the ORCID record for Maria Olívia Mercadante-Simões [Opens in a new window]
Wanessa Stéfanne de Jesus Silva Bruzinga
Affiliation:
Departamento de Biologia Geral, Universidade Estadual de Montes Claros, Montes Claros, MG 39401-089, Brazil
Leonardo Monteiro Ribeiro
Affiliation:
Departamento de Biologia Geral, Universidade Estadual de Montes Claros, Montes Claros, MG 39401-089, Brazil
Yule Roberta Ferreira Nunes
Affiliation:
Departamento de Biologia Geral, Universidade Estadual de Montes Claros, Montes Claros, MG 39401-089, Brazil
Lúcia Pinheiro Santos Pimenta
Affiliation:
Departamento de Química, Universidade Federal de Minas Gerais, Belo Horizonte, MG 31270-901, Brazil
Mariana Araujo Thibau de Almeida
Affiliation:
Departamento de Química, Universidade Federal de Minas Gerais, Belo Horizonte, MG 31270-901, Brazil
Martha Grasielle Alves Mendes
Affiliation:
Departamento de Biologia Geral, Universidade Estadual de Montes Claros, Montes Claros, MG 39401-089, Brazil
Maria Olívia Mercadante-Simões*
Affiliation:
Departamento de Biologia Geral, Universidade Estadual de Montes Claros, Montes Claros, MG 39401-089, Brazil
*
*Corresponding author: Maria Olívia Mercadante-Simões, E-mail: [email protected]
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Abstract

The objective of this work was to characterize the ontogenesis of Protium spruceanum secretory ducts, to evaluate the effects of seasonality on that process, and to characterize the chemical nature of the resin. Morphometric, anatomical, micromorphometric, histochemical, and ultrastructural evaluations of shoot apexes and chemical analyses of the resin were performed. The ducts of schizolysigenous origin are distributed in the primary and secondary phloem. The subsecretory tissue is meristematic and can restore the secretory epithelium. Secretory epithelial cells have wall thickening resembling that of the Casparian strip that regulates secretion reflux. The main resin compounds are pentacyclic triterpenoids, α- and β-amyrins, and α- and β-amyrenones, which are reported here for the first time for this species. The presence of electron-dense and electron-opaque structures, in the secretory epithelial cells, are compatible with the triterpenes and mucilage identified in the resin. Rising temperatures, rainfall, and increasing day length induce the formation of ducts in the vascular cambium throughout Spring/Summer. The abundant production of resin rich in pentacyclic triterpenes indicates the potential use of the species for medicinal and cosmetic purposes. The understanding that secretory processes are concentrated during the Spring/Summer seasons will contribute to the definition of resin extraction management strategies.

Keywords

apical meristemα- and β-amyrinsα- and β-amyrenonesschizolisygenous ductsvascular cambium
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 28 , Issue 3 , June 2022 , pp. 744 - 755
Copyright
Copyright © The Author(s), 2022. Published by Cambridge University Press on behalf of the Microscopy Society of America

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