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A Novel Technique to Determine Platelet Deposition on an Experimental Nylon Membrane Using Lvsem and Tem

Published online by Cambridge University Press:  02 July 2020

M.A. Murphy  and
J.P. DiOrio
M.A. Murphy
Affiliation:
Baxter International Inc, Round Lake, IL60073
J.P. DiOrio
Affiliation:
Baxter International Inc, Round Lake, IL60073
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Extract

Porous nylon fabric is used as a filter in blood devices. This study was undertaken to evaluate the interaction between an experimental nylon filter and the cellular elements which pass through it. Our investigation was mainly centered on determining the deposition of cellular material on the nylon filter using Low Voltage Scanning Electron Microscopy (LVSEM) and Transmission Electron Microscopy (TEM).

The filter was prepared for LVSEM by cutting a 2.5 cm2 of the nylon filter material with a razor and placing it in a nylon mesh bag which was stapled shut. The bag was rinsed three times for ten minutes each in fresh Sorensen's phosphate buffer. A graded alcohol series of 25%, 35%, 50%, 70%, 80%, and 90% was used to dehydrate the filter for ten minutes each. This was followed by dehydration twice for 20 minutes in fresh 100% ETOH. The filter was then Critical Point Dried (CPD) in the Tousimis AUTOSAMDRI-814.

Type
Specimen Preparation
Information
Microscopy and Microanalysis , Volume 4 , Issue S2: Proceedings: Microscopy & Microanalysis '98, Microscopy Society of America 56th Annual Meeting, Microbeam Analysis Society 32nd Annual Meeting, Atlanta, Georgia July 12-16, 1998 , July 1998 , pp. 848 - 849
Copyright
Copyright © Microscopy Society of America

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References

1. Sato, T.. Journal ofElectron Microscopy, 14(1968)158.Google Scholar