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Novel Methods in Fluorescence Sensing

Published online by Cambridge University Press:  02 July 2020

Joseph R. Lakowicz ,
Ignacy Gryczynski ,
Zygmunt Gryczynski ,
Leah Tolosa ,
Govind Rao ,
Jonathan Dattelbaum  and
Lisa Eichorn
Joseph R. Lakowicz
Affiliation:
Center for Fluorescence Spectroscopy, University of Maryland at Baltimore, School of Medicine, Department of Biochemistry and Molecular Biology, 725 West Lombard Street, Baltimore, Maryland21201
Ignacy Gryczynski
Affiliation:
Center for Fluorescence Spectroscopy, University of Maryland at Baltimore, School of Medicine, Department of Biochemistry and Molecular Biology, 725 West Lombard Street, Baltimore, Maryland21201
Zygmunt Gryczynski
Affiliation:
Center for Fluorescence Spectroscopy, University of Maryland at Baltimore, School of Medicine, Department of Biochemistry and Molecular Biology, 725 West Lombard Street, Baltimore, Maryland21201
Leah Tolosa
Affiliation:
Center for Fluorescence Spectroscopy, University of Maryland at Baltimore, School of Medicine, Department of Biochemistry and Molecular Biology, 725 West Lombard Street, Baltimore, Maryland21201
Govind Rao
Affiliation:
Center for Fluorescence Spectroscopy, University of Maryland at Baltimore, School of Medicine, Department of Biochemistry and Molecular Biology, 725 West Lombard Street, Baltimore, Maryland21201
Jonathan Dattelbaum
Affiliation:
Center for Fluorescence Spectroscopy, University of Maryland at Baltimore, School of Medicine, Department of Biochemistry and Molecular Biology, 725 West Lombard Street, Baltimore, Maryland21201
Lisa Eichorn
Affiliation:
Center for Fluorescence Spectroscopy, University of Maryland at Baltimore, School of Medicine, Department of Biochemistry and Molecular Biology, 725 West Lombard Street, Baltimore, Maryland21201
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Extract

Chemical sensing based on fluorescence is presently undergoing rapid development. As one example, fluorescence lifetime measurements are now being used for measuring cation and anion concentrations in clinical chemistry, cellular imaging and flow cytometry. Additionally, there is an increasing use of proteins as sensors because of their high affinity and specificity for ligands.

In this report we describe some recently developed approaches to fluorescence sensing. These new methods are based on the use of a fluorescence reference either within the sample or external to the sample, but within the optical path. Two types of references are used; microsecond lifetime metalligand complexes (MLCs) or oriented films of ns decay time fluorophores. A protein sensor for glucose was developed using the glucose-galactose binding protein (GGBP) from E.coli (Figure 1).

Type
Molecular Optical Spectroscopy in Biology
Information
Microscopy and Microanalysis , Volume 5 , Issue S2: Proceedings: Microscopy & Microanalysis '99, Microscopy Society of America 57th Annual Meeting, Microbeam Analysis Society 33rd Annual Meeting, Portland, Oregon August 1-5, 1999 , August 1999 , pp. 506 - 507
Copyright
Copyright © Microscopy Society of America

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