Hostname: page-component-586b7cd67f-2brh9 Total loading time: 0 Render date: 2024-11-28T19:52:51.942Z Has data issue: false hasContentIssue false
  • English
  • Français

A New Smaller Sized Virus-Like Particle in Drosophila Melanogaster

Published online by Cambridge University Press:  02 July 2020

Jeffrey G. Ault  and
Ellen Shimakawa
Jeffrey G. Ault
Affiliation:
Division of Molecular Medicine, Wadsworth Center, New York State Department of Health, Albany, NY, 12201-0509, USA
Ellen Shimakawa
Affiliation:
Biology Department, Chaminade University, Honolulu, HI, 96816, USA
Get access

Abstract

During a chromosome behavior study involving high-pressure freezing (HPF)/freeze substitution (FS) of Drosophila melanogaster testes, we discovered quasi-crystalline inclusions in the nuclei of adjacent gut epithelial cells (Fig. 1). The HPF and FS protocols were standard. The viscera of adult flies were packed in yeast paste for HPF. The tissue was fixed by FS with 1% osmium tetroxide in acetone for 72 hours at -90° C then 48 hours at -60° C. Afterwards, it was washed several times at room temperature in 100% acetone and embedded in Epon/Araldite. Thin sections were cut and stained with uranyl acetate and lead. As expected with HPF/FS, the material was well-preserved with straight microtubules, smooth membranes, dense mitochondria, and abundant ribosomes both on the rough endoplasmic reticulum and in the full cytoplasm (Fig. 1).

The inclusions consisted of virus-like particles packed loosely together in orderly arrays. Particles were usually hexagonally packed with spaces disrupting the periodicity (Figs. 2 and 3).

Type
Microbiology
Information
Microscopy and Microanalysis , Volume 7 , Issue S2: Proceedings: Microscopy & Microanalysis 2001, Microscopy Society of America 59th Annual Meeting, Microbeam Analysis Society 35th Annual Meeting, Long Beach, California August 5-9, 2001 , August 2001 , pp. 742 - 743
Copyright
Copyright © Microscopy Society of America 2001

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

1.McDonald, K. and Morphew, M. K., Microsc. Res. Tech. 24(1993)465.CrossRefGoogle Scholar
2.Akai, H. et al, Sci. 157(1967)810.CrossRefGoogle Scholar
3.Filshie, B. K. et al., J. Invert. Path. 9(1967)271.CrossRefGoogle Scholar
4.Philpott, D. E. et al., J. Invert. Path. 14(1969)31.CrossRefGoogle Scholar
5.Gartner, L. P., Experientia 27(1971)562.CrossRefGoogle Scholar
6.Felluga, B. et al., J. Invert. Path. 17(1971)339.CrossRefGoogle Scholar
7.This work was done at the Wadsworth Center's EM Core facility. We would like to thank itsdirector, William Samsonoff, for his support and knowledge of viruses.Google Scholar