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A New EDS Equipment (GEM Detector With Superatw Window) and a Correction for Si-Contamination Improve the Quantitative Electron Probe Micro Analysis of Biological Cryosections

Published online by Cambridge University Press:  02 July 2020

M Schultz ,
F. Rudolf  and
M.F. Wendt-Gallitelli
M Schultz
Affiliation:
Department of Physiology, University of Halle, Magdeburger Str.6, D-06097 Halle/Saale
F. Rudolf
Affiliation:
Department of Physiology, University of Halle, Magdeburger Str.6, D-06097 Halle/Saale
M.F. Wendt-Gallitelli
Affiliation:
Department of Physiology, University of Halle, Magdeburger Str.6, D-06097 Halle/Saale
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Extract

Function dependent changes in the intracellular (i.c.) concentrations of calcium and sodium play a crucial role in the regulation of biological processes such as contraction of heart muscle. However, accurate measurements of both elements are difficult since in i.e. compartments they are present in low concentrations, hence the Ca and Na peaks show small count rates and small peak to continuum ratios. The accurate quantification of Ca and Na is rendered even more difficult by the partially overlap of the NaK with CuL peak and CaKα with KKβ peak. In some cases electrophysiologist substitute in the experiments K+ with Cs+ or the pathologist is interested in the storage of mercury. For analysis of such elements (Cs, Hg) with higher atomic number the stopping power of the EDS crystal play an important role for system sensitivity. Quantitative analysis of thin biological cryosections with the continuum method must be corrected for influence of X-rays emit from holder, supporting film and grid.

Type
30 Years of Energy Dispersive Spectrometry in Microanalysis
Information
Microscopy and Microanalysis , Volume 4 , Issue S2: Proceedings: Microscopy & Microanalysis '98, Microscopy Society of America 56th Annual Meeting, Microbeam Analysis Society 32nd Annual Meeting, Atlanta, Georgia July 12-16, 1998 , July 1998 , pp. 200 - 201
Copyright
Copyright © Microscopy Society of America

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References

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7. This research was supported by the Deutsche Forschungsgemeinschaft, Grant We 789/5-1 and BMBF 313005 to M.F.W.G.. The authors thank Nicole Walter for excellent technical assistance.Google Scholar