Hostname: page-component-586b7cd67f-dsjbd Total loading time: 0 Render date: 2024-12-01T11:43:59.670Z Has data issue: false hasContentIssue false
  • English
  • Français

New Concept in Helium Cooled Transmission Microscopes Philips Tecnai F30 Helium: Design and First Results

Published online by Cambridge University Press:  02 July 2020

U. Lücken ,
R. Wagner ,
AF de Jong  and
W. Hax
U. Lücken
Affiliation:
FEI Electron Optics, PO Box 218, 5600, MD, Eindhoven, The Netherlands
R. Wagner
Affiliation:
FEI Electron Optics, PO Box 218, 5600, MD, Eindhoven, The Netherlands
AF de Jong
Affiliation:
FEI Electron Optics, PO Box 218, 5600, MD, Eindhoven, The Netherlands
W. Hax
Affiliation:
FEI Electron Optics, PO Box 218, 5600, MD, Eindhoven, The Netherlands
Get access

Abstract

To date it has been possible to solve the structure of several membrane proteins to atomic resolution with cryo-electron crystallography (1-4). in all studies two-dimensional crystals were required as well as special provisions on the transmission microscope. in all cases a field emission gun provided the necessary coherence and liquid-Helium cooling the specimen protection (4,5).

Although repeatedly studies have been done on the cryo-protection factor of beam sensitive specimen at liquid-Nitrogen and liquid-Helium temperatures (6-10), the data available are still too limited for a clear demonstration of the superiority of liquid-Helium. Especially investigations are needed with individual particles embedded in amorphous ice (11).

To meet the increasing demand for liquid-Helium cooling, FEI developed the Tecnai F30 Helium. The challenge was to provide an instrument with a very stable cryo-stage, which can work at liquid- Nitrogen as well as liquid-Helium temperature.

Type
Corporate Session (Organized by M. Kersker)
Information
Microscopy and Microanalysis , Volume 7 , Issue S2: Proceedings: Microscopy & Microanalysis 2001, Microscopy Society of America 59th Annual Meeting, Microbeam Analysis Society 35th Annual Meeting, Long Beach, California August 5-9, 2001 , August 2001 , pp. 868 - 869
Copyright
Copyright © Microscopy Society of America 2001

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

references

1.Henderson, R.et al.J. Mol. Biol. 1990 213:899CrossRefGoogle Scholar
2.Kühlbrandt, W.et al.Nature 1994 367:614CrossRefGoogle Scholar
3.Walz, T.et al.Nature 1997 387:624CrossRefGoogle Scholar
4.Fujiyoshi, Y., Adv. Biophys. 1998 35:25CrossRefGoogle Scholar
5.Zemlin, F.et al.Ultramicroscopy 1996 63:125CrossRefGoogle Scholar
6.Hayward, et al.Ultramicroscopy 1979 4:201CrossRefGoogle Scholar
7.Unwin, N.et al.J. Mol. Biol. 1975 94:425CrossRefGoogle Scholar
8.Chiu, et al.Ultramicroscopy 1982 10:63CrossRefGoogle Scholar
9.Knapek, etal.Ultramicroscopy 1982 10:105CrossRefGoogle Scholar
10.Stark, et al.Ultramicroscopy 1996 63:75CrossRefGoogle Scholar
11.Van, Heel, Current Opinions in Structural Biology 2000 10:259Google Scholar