Hostname: page-component-586b7cd67f-t7fkt Total loading time: 0 Render date: 2024-12-01T04:06:56.545Z Has data issue: false hasContentIssue false
  • English
  • Français

Neutrophil Microtubules Display Unusual Properties and are Highly Sensitive to Taxol

Published online by Cambridge University Press:  02 July 2020

J.M. Robinson  and
D.D. Vandré
J.M. Robinson
Affiliation:
Department of Cell Biology, Neurobiology, and Anatomy, Ohio State University, Columbus, OH43210
D.D. Vandré
Affiliation:
Department of Cell Biology, Neurobiology, and Anatomy, Ohio State University, Columbus, OH43210
Get access

Extract

The microtubule (MT) cytoskeleton plays a crucial role in a number of cellular functions including cytoplasmic organization, intracellular transport, cellular polarity, and cell division. Microtubules are linear polymeric structures composed of heterodimers of α- and β-tubulin with a class of specialized proteins, the microtubule-associated proteins, typically being present. Microtubules are dynamic structures that undergo repeated rounds of assembly/disassembly. The half-life of MTs, an index of their dynamics (i.e., assembly-disassembly), has been estimated from studies of single cells in culture and typically ranges from ≈ 5 - 20 minutes depending on the cell type. These dynamic properties of MTs have been explained in terms of the “dynamic instability” model. Five parameters have been used to describe dynamic instability of MTs: (1) elongation velocity; (2) shortening velocity; (3) nucleation frequency; (4) catastrophe frequency; and (5) rescue frequency. Catastrophe is the transition from elongation to shortening while rescue is the transition from shortening to elongation.

Type
Chemotherapeutic Agents That Affect Microtubules: Mechanisms of Response and Chemotherapeutic Agents and Microtubules
Information
Microscopy and Microanalysis , Volume 4 , Issue S2: Proceedings: Microscopy & Microanalysis '98, Microscopy Society of America 56th Annual Meeting, Microbeam Analysis Society 32nd Annual Meeting, Atlanta, Georgia July 12-16, 1998 , July 1998 , pp. 1038 - 1039
Copyright
Copyright © Microscopy Society of America

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

1.Gelfand, V.I. and Bershadsky, A.R.. Annu. Rev. Cell Biol. 7 (1991)93.CrossRefGoogle Scholar
2.Cassimeris, L. et al., J. Cell Biol. 102 (1986)2023.Google Scholar
3.Mitchison, T. and Kirschner, M.W.. Nature. 312(1984)237.Google Scholar
4.Walker, R.A. et al., J. Cell Biol. 107 (1988)CrossRefGoogle Scholar
5.Schliwa, M. et al., Cell. 31 (1982)705.CrossRefGoogle ScholarPubMed
6.Ding, M. et al., Eur. J. Cell Biol. 66(1995)234.Google Scholar
7.Robinson, J.M. and Vandre, D.D.. J. Histochem. Cytochem. 45 (1997)631.CrossRefGoogle Scholar
8.Wilson, L. and Jordan, M.A.. Chemistry & Biology. 2(1995)569.CrossRefGoogle Scholar
9.Cassimeris, L.. CellMotil. Cytoskel. 26 (1993)275.CrossRefGoogle Scholar
10.Wadsworth, P. and McGrail, M.. J. CellSci. 95 (1990)23.Google Scholar
11.Robinson, J.M. and Vandre, D.D.. J. Cell Sci. 108 (1995)645.CrossRefGoogle Scholar