Hostname: page-component-586b7cd67f-t7czq Total loading time: 0 Render date: 2024-11-27T23:02:26.614Z Has data issue: false hasContentIssue false
  • English
  • Français

Nanoleakage in Hybrid Layer and Acid–Base Resistant Zone at the Adhesive/Dentin Interface

Published online by Cambridge University Press:  09 September 2015

Toru Nikaido ,
Hamid Nurrohman ,
Tomohiro Takagaki ,
Alireza Sadr ,
Shizuko Ichinose  and
Junji Tagami
Toru Nikaido*
Affiliation:
Department of Cariology and Operative Dentistry, Division of Oral Health Sciences, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University (TMDU), 1-5-45, Yushima, Bunkyo-ku, Tokyo 113-8549, Japan
Hamid Nurrohman
Affiliation:
Department of Preventive and Restorative Dental Sciences, University of California, Box 0758, 707 Parnassus Ave., San Francisco, CA 94143-0758, USA
Tomohiro Takagaki
Affiliation:
Department of Cariology and Operative Dentistry, Division of Oral Health Sciences, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University (TMDU), 1-5-45, Yushima, Bunkyo-ku, Tokyo 113-8549, Japan
Alireza Sadr
Affiliation:
Biomimetics Biomaterials Biophotonics & Technology Laboratory, Department of Restorative Dentistry, University of Washington School of Dentistry; 1959 NE Pacific St., Box 357456, Seattle, WA 98195-7456, USA
Shizuko Ichinose
Affiliation:
Instrumental Analysis Research Center, Tokyo Medical and Dental University (TMDU); 1-5-45, Yushima, Bunkyo-ku, Tokyo 113-8549, Japan
Junji Tagami
Affiliation:
Department of Cariology and Operative Dentistry, Division of Oral Health Sciences, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University (TMDU), 1-5-45, Yushima, Bunkyo-ku, Tokyo 113-8549, Japan
*
*Corresponding author.[email protected]
Get access

Abstract

The aim of interfacial nanoleakage evaluation is to gain a better understanding of degradation of the adhesive–dentin interface. The acid–base resistant zone (ABRZ) is recognized at the bonded interface under the hybrid layer (HL) in self-etch adhesive systems after an acid–base challenge. The purpose of this study was to evaluate nanoleakage in HL and ABRZ using three self-etch adhesives; Clearfil SE Bond (SEB), Clearfil SE One (SEO), and G-Bond Plus (GBP). One of the three adhesives was applied on the ground dentin surface and light cured. The specimens were longitudinally divided into two halves. One half remained as the control group. The others were immersed in ammoniacal silver nitrate solution, followed by photo developing solution under fluorescent light. Following this, the specimens were subjected to acid–base challenges with an artificial demineralization solution (pH4.5) and sodium hypochlorite, and prepared in accordance with common procedures for transmission electron microscopy (TEM) examination. The TEM images revealed silver depositions in HL and ABRZ due to nanoleakage in all the adhesives; however, the extent of nanoleakage was material dependent. Funnel-shaped erosion beneath the ABRZ was observed only in the all-in-one adhesive systems; SEO and GBP, but not in the two-step self-etch adhesive system; SEB.

Keywords

dentin bondingself-etch systemTEMHLacid–base resistant zonenanoleakage
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 21 , Issue 5 , October 2015 , pp. 1271 - 1277
Copyright
© Microscopy Society of America 2015 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Coutinho, E., Cardoso, M.V., Fernandes, C.P., Neves, A.A., Gouvea, C.V., Van Landuyt, K.L., De Munck, J. & Van Meerbeek, B. (2011). Nanoleakage distribution at adhesive-dentin interfaces in 3D. J Dent Res 90, 1019–1025.Google ScholarPubMed
De Munck, J., Van Meerbeek, B., Yoshida, Y., Inoue, S., Vargas, M., Suzuki, K., Lambrechts, P. & Vanherle, G. (2003). Four-year water degradation of total-etch adhesives bonded to dentine. J Dent Res 82, 136–140.Google Scholar
Hariri, I., Shimada, Y., Sadr, A., Ichinose, S. & Tagami, J. (2012). The effects of aging on shear bond strength and nanoleakage expression of an etch-and-rinse adhesive on human enamel and dentin. J Adhes Dent 14, 235–243.Google ScholarPubMed
Hashimoto, M., Fujita, S., Endo, K. & Ohno, H. (2009). In vitro degradation of resin-dentin bonds with one-bottle self-etching adhesives. Eur J Oral Sci 117, 611–617.Google ScholarPubMed
Hashimoto, M., Ohno, H., Sano, H., Tay, F.R., Kaga, M., Kudou, Y., Oguchi, H., Araki, Y. & Kubota, M. (2002). Micromorphological changes in resin-dentin bonds after 1 year of water storage. J Biomed Mater Res 63, 306–311.Google ScholarPubMed
Ichinose, S., Muneta, T., Aoki, H. & Tagami, M. (2003). TEM observation of seven retrieved total knee joints made of Corimary too Tiven retrieved. Biomed Mater Eng 13, 125–134.Google Scholar
Iida, Y., Nikaido, T., Kitayama, S., Takagaki, T., Inoue, G., Ikeda, M., Foxton, R.M. & Tagami, J. (2009). Evaluation of dentin bonding performance and acid-base resistance of the interface of two-step self-etching adhesive systems. Dent Mater J 28, 493–500.Google Scholar
Inoue, G., Nikaido, T., Foxton, R.M. & Tagami, J. (2009). The acid-base resistant zone in three dentin bonding systems. Dent Mater J 28, 717–721.Google Scholar
Inoue, G., Nikaido, T., Sadr, A. & Tagami, J. (2012). Morphological categorization of acid-base resistant zones with self-etching primer adhesive systems. Dent Mater J 31, 232–238.Google Scholar
Inoue, G., Tsuchiya, S., Nikaido, T., Foxton, R.M. & Tagami, J. (2006). Morphological and mechanical characterization of the acid-base resistant zone at the adhesive-dentin interface of intact and caries-affected dentin. Oper Dent 31, 466–472.Google ScholarPubMed
Li, N., Nikaido, T., Takagaki, T., Sadr, A., Makishi, P., Chen, J. & Tagami, J. (2010). The role of functional monomers in bonding to enamel: acid-base resistant zone and bonding performance. J Dent 38, 722–730.Google Scholar
Li, N., Takagaki, T., Sadr, A., Waidyasekera, K., Ikeda, M., Chen, J. Nikaido, T. & Tagami, J. (2011). Effect of curing modes of dual-curing core systems on microtensile bond strength to dentin and formation of an acid-base resistant zone. J Adhes Dent 13, 527–535.Google Scholar
Milia, E., Cumbo, E., Cardoso, R.J. & Gallina, G. (2012). Current dental adhesives systems. A narrative review. Curr Pharm Des 18, 5542–5552.Google Scholar
Nakabayashi, N., Kojima, K. & Masuhara, E. (1982). The promotion of adhesion by the infiltration of monomers into tooth substrates. J Biomed Mater Res 16, 265–273.Google ScholarPubMed
Nikaido, T., Inoue, G., Takagaki, T., Waidyasekera, K., Iida, Y., Shinohara, S.M., Sadr, A. & Tagami, J. (2011). New strategy to create “Super Dentin” using adhesive technology: reinforcement of adhesive-dentin interface and protection of tooth structures. Jpn Dent Sci Rev 47, 31–42.Google Scholar
Nikaido, T., Weerashinghe, D.D.S., Waidyasekera, K., Inoue, G., Foxton, R.M. & Tagami, J. (2009). Assessment of the nanostructure of acid-base resistant zone by the application of all-in-one adhesive systems: super dentin formation. Biomed Mater Eng 19, 163–171.Google ScholarPubMed
Nurrohman, H., Nikaido, T., Takagaki, T., Sadr, A., Ichinose, S. & Tagami, J. (2012 a) Hydroxyapatite crystal protection against acid-attack beneath resin-dentin interface with four adhesives: TEM and crystallography evidence. Dent Mater 28, e89–e98.Google Scholar
Nurrohman, H., Nikaido, T., Takagaki, T., Sadr, A., Waidyasekera, K., Kitayama, S., Ikeda, M. & Tagami, J. (2012 b) Dentin bonding performance and ability of four MMA-based adhesive resins to prevent demineralization along the hybrid layer. J Adhes Dent 14, 339–348.Google Scholar
Sano, H., Yoshikawa, T., Pereira, P.N.R., Kanemura, N., Morigami, M., Tagami, J. & Pashley, D.H. (1999). Long-term durability of dentin bonds made with a self-etching primer, in vivo. J Dent Res 78, 906–911.Google Scholar
Sano, H., Yoshiyama, M., Ebisu, S., Burrow, M.F., Takatsu, T., Ciucchi, B., Carvalho, R. & Pashley, D.H. (1995). Comparative SEM and TEM observations of nanoleakage within the hybrid layer. Oper Dent 20, 160–167.Google Scholar
Tay, F.R., Pashley, D.H. & Yoshiyama, M. (2002). Two modes of nanoleakage expression in single-step adhesives. J Dent Res 81, 472–476.Google Scholar
Tjäderhane, L., Nascimento., F.D., Breschi, L., Mazzoni, A., Tersariol, I.L., Geraldeli, S., Tezvergil-Mutluay, A., Carrilho, M.R., Carvalho, R.M., Tay, F.R. & Pashley, D.H. (2013). Optimizing dentin bond durability: control of collagen degradation by matrix metalloproteinases and cysteine cathepsins. Dent Mater 29, 116–135.Google ScholarPubMed
Tsuchiya, S., Nikaido, T., Sonoda, H., Foxton, R.M. & Tagami, J. (2004). Ultrastructure of the dentin-adhesive interface after acid-base challenge. J Adhes Dent 6, 183–190.Google Scholar
Tsujimoto, M., Nikaido, T., Inoue, G., Sadr, A. & Tagami, J. (2010). Ultrastructural observations of the acid-base resistant zone of all-in-one adhesives using three different acid-base challenges. Dent Mater J 29, 655–660.Google Scholar
Van Landuyt, K.L., Yoshida, Y., Hirata, I., Snauwaert, J., De Munck, J., Okazaki, M., Suzuki, K., Lambrechts, P. & Van Meerbeek, B. (2008). Influence of the chemical structure of functional monomers on their adhesive performance. J Dent Res 87, 757–761.Google Scholar
Van Meerbeek, B., Yoshihara, K., Yoshida, Y., Mine, A., De Munck, J. & Van Lauduyt, K.L. (2010). State of the art of self-etch adhesives. Dent Mater 27, 17–28.Google Scholar
Waidyasekera, K., Nikaido, T., Weerasinghe, D.S., Ichinose, S. & Tagami, J. (2009). Reinforcement of dentin in self-etch adhesive technology: a new concept. J Dent 37, 604–609.Google Scholar
Wefel, J.S., Heilman, J.R. & Jordan, T.H. (1995). Comparisons of in vitro root caries models. Caries Res 29, 204–209.Google ScholarPubMed
Yoshida, Y., Nagakane, K., Fukuda, R., Nakayama, Y., Okazaki, M., Shintani, H., Inoue, S., Tagawa, Y., Suzuki, K., De Munck, J. & Van Meerbeek, B. (2004). Comparative study on adhesive performance of functional monomers. J Dent Res 83, 454–458.Google Scholar
Yoshihara, K., Yoshida, Y., Nagaoka, N., Fukegawa, D., Hayakawa, S., Mine, A., Nakamura, M., Minagi, S., Osaka, A., Suzuki, K. & Van Meerbeek, B. (2010). Nano-controlled molecular interaction at adhesive interfaces for hard tissue reconstruction. Acta Biomater 6, 3573–3582.Google ScholarPubMed