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Myotendinous Junction Components of Different Skeletal Muscles Present Morphological Changes in Obese Rats

Published online by Cambridge University Press:  21 April 2021

Bruna Aléxia Cristofoletti Grillo Open the ORCID record for Bruna Aléxia Cristofoletti Grillo [Opens in a new window] ,
Lara C. Rocha Open the ORCID record for Lara C. Rocha [Opens in a new window] ,
Giovana Z. Martinez ,
Jurandyr Pimentel Neto Open the ORCID record for Jurandyr Pimentel Neto [Opens in a new window] ,
Carolina dos Santos Jacob Open the ORCID record for Carolina dos Santos Jacob [Opens in a new window] ,
Ii-sei Watanabe Open the ORCID record for Ii-sei Watanabe [Opens in a new window]  and
Adriano P. Ciena Open the ORCID record for Adriano P. Ciena [Opens in a new window]
Bruna Aléxia Cristofoletti Grillo
Affiliation:
Laboratory of Morphology and Physical Activity (LAMAF), Institute of Biosciences (IB), São Paulo State University (UNESP), Rio Claro, SP13506-900, Brazil
Lara C. Rocha*
Affiliation:
Laboratory of Morphology and Physical Activity (LAMAF), Institute of Biosciences (IB), São Paulo State University (UNESP), Rio Claro, SP13506-900, Brazil
Giovana Z. Martinez
Affiliation:
Laboratory of Morphology and Physical Activity (LAMAF), Institute of Biosciences (IB), São Paulo State University (UNESP), Rio Claro, SP13506-900, Brazil
Jurandyr Pimentel Neto
Affiliation:
Laboratory of Morphology and Physical Activity (LAMAF), Institute of Biosciences (IB), São Paulo State University (UNESP), Rio Claro, SP13506-900, Brazil
Carolina dos Santos Jacob
Affiliation:
Laboratory of Morphology and Physical Activity (LAMAF), Institute of Biosciences (IB), São Paulo State University (UNESP), Rio Claro, SP13506-900, Brazil
Ii-sei Watanabe
Affiliation:
Department of Anatomy, Institute of Biomedical Sciences -ICB III, University of São Paulo (USP), São Paulo, SP05508-900, Brazil
Adriano P. Ciena
Affiliation:
Laboratory of Morphology and Physical Activity (LAMAF), Institute of Biosciences (IB), São Paulo State University (UNESP), Rio Claro, SP13506-900, Brazil
*
*Author for correspondence: Lara C. Rocha, E-mail: [email protected]
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Abstract

Obesity is characterized by excess adipose tissue and chronic inflammation and promotes extensive changes that can compromise skeletal muscles’ structural and functional integrity. Obesity can seriously impact the force transmission region between the muscle and the tendon, the myotendinous junction (MTJ). The present study aimed to investigate the plasticity of muscle fibers and MTJ regions in high-fat diet-induced obesity in rat tibialis anterior (TA) and soleus (SO) muscles. Wistar rats were divided into control and obese groups (induced by a high-fat diet). The samples of TA and SO muscles were prepared for histochemical and ultrastructural analysis (sarcomeres and MTJ projection). In the muscle fiber, similar adaptations were observed between the muscles of the smaller fiber (types I and IIa) in the obesity results. The MTJ region demonstrated different adaptations between the analyzed muscles. The TA–MTJ region has shorter ultrastructures, while in the SO–MTJ region, the ultrastructures were larger. We conclude that obesity induced by a high-fat diet promotes similar adaptation in the muscle fibers; however, in the MTJ region, the sarcoplasmatic projections and adjacent sarcomere demonstrate different adaptations according to distinct muscle phenotypes.

Keywords

extracellular matrixmyotendinous junctionobesitysarcomeresupport collagen layer
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 27 , Issue 3 , June 2021 , pp. 598 - 603
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Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press on behalf of the Microscopy Society of America

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