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Multi-Dimensional Microscopic Analysis of Sea Urchin Development

Published online by Cambridge University Press:  02 July 2020

R. Summers ,
G. Martins  and
J. Morrill
R. Summers
Affiliation:
Dept. Cell Biology, Scripps Research Institute, La Jolla, CA92037
G. Martins
Affiliation:
Dept. Anatomy and Cell Biology, SUNY Buffalo, Buffalo, NY14214
J. Morrill
Affiliation:
Div. Nat. Sciences, New College-USF, Sarasota, FL34243.
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Extract

Several modalities of microscopic analysis have been employed in the investigation of development in the embryos of the sea urchins Strongylocentrotus purpuratusand Lytechinusvariegatus. These include scanning electron microscopy, confocal optical microscopy and multiphoton laser scanning microscopy. These techniques all provide information on embryonic morphogenesis in three-dimensions and may be of use in the analysis of other developing organisms.

Scanning Electron Microscopy. Scanning electron microscopy bridges the gap between light and transmission electron microscopy. Operation in the secondary electron image mode with stereo-pair images combines great depth of field in focus from 10-50,000x with true 3D images of topographical features from whole embryos to macromolecules. The SEM's backscatter electron imaging mode allows one to see and map spatial geographies of colloidal gold labeled probes (e.g. antibodies, lectins) that bind to exposed surfaces of embryos, cells and extracellular matrices.

Type
Applications of Imaging Techniques to the Study of Embryological Development
Information
Microscopy and Microanalysis , Volume 5 , Issue S2: Proceedings: Microscopy & Microanalysis '99, Microscopy Society of America 57th Annual Meeting, Microbeam Analysis Society 33rd Annual Meeting, Portland, Oregon August 1-5, 1999 , August 1999 , pp. 1068 - 1069
Copyright
Copyright © Microscopy Society of America

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References

1 . Morrill, J. B., Methods in Cell Biology, San Diego Academic Press 27 (1986) 263.Google Scholar

2 . Cherr, et al., Microsc. Res. Tech. 22 (1992) 11.CrossRefGoogle Scholar

3 . Summers, R.G. and Cheng, P.C., Proc. 47th Ann Meeting EMSA (1989) 140.CrossRefGoogle Scholar

4 . Cheng, P.C. and Summers, R.G., The Handbook of Biological Confocal Microscopy, San Francisco Press (1989) 163.Google Scholar

5 . Summers, R.G. et al., J. Electron Micr. Tech. 18 (1991) 24.CrossRefGoogle Scholar

6 . Summers, R.G. et al., Dev. Growth Diff 35 (1991) 41.CrossRefGoogle Scholar

7 . Summers, R.G. et al., Methods in Cell Biology, San Diego Academic Press 38 (1993) 265.Google Scholar

8 . Marko, M. et al., J. Electron Microsc. Technol. 9 (1988) 395.CrossRefGoogle Scholar

9 . Martins, G. G et al, Develop. Biol. 198 (1998) 330.Google Scholar

10. Summers, R G. et al., Develop. Biol. 175 (1996) 777.CrossRefGoogle Scholar

11. Piston, D. W. et al., Microsc. Microanal. 4 (1998) 404.CrossRefGoogle Scholar