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A Morphometric Analysis of Early Effects of Ethanol on Hepatocyte Organelles from Peromyscusmaniculatus

Published online by Cambridge University Press:  02 July 2020

J. T. Ellzey ,
J. P. Drake ,
L. Dader  and
P. Boentges
J. T. Ellzey
Affiliation:
Department of Biological Sciences, University of Texas at El Paso, El Paso, TX79968-0519
J. P. Drake
Affiliation:
Department of Biological Sciences, University of Texas at El Paso, El Paso, TX79968-0519
L. Dader
Affiliation:
Department of Biological Sciences, University of Texas at El Paso, El Paso, TX79968-0519
P. Boentges
Affiliation:
Department of Biological Sciences, University of Texas at El Paso, El Paso, TX79968-0519
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Extract

Pathological changes of hepatocytes from rats fed a 30% ethanol-derived calories diet for three weeks include noticeable ultrastructural changes including steatosis and hypertrophy of the smooth endoplasmic reticulum. We sought to examine hepatocytes of deer mice administered ethanol in an inhalation chamber for two weeks to determine if subtle changes occur in hepatocyte organelles prior to steatosis.

Two strains of Peromyscus maniculatus, ADH-positive possessing hepatic cytosolic alcohol dehydrogenase and ADH-negative deer mice lacking this enzyme were purchased from the Peromyscus Genetic Stock Center (Univ. of South Carolina). They tested negatively for Hanta viruses. A morphometric analysis of the ultrastructure of ADH+(n=14) and ADH- (n=14) controls as well as experimentals exposed to chronic, intoxicating levels of ethanol was conducted. Blood ethanol levels were maintained between 1.25-1.75 mg/ml for two weeks in the experimentals.

Type
Pathology
Information
Microscopy and Microanalysis , Volume 4 , Issue S2: Proceedings: Microscopy & Microanalysis '98, Microscopy Society of America 56th Annual Meeting, Microbeam Analysis Society 32nd Annual Meeting, Atlanta, Georgia July 12-16, 1998 , July 1998 , pp. 1064 - 1065
Copyright
Copyright © Microscopy Society of America

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References

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3. This research was undertaken in the Analytical Cytology Core Facility supported by NIH grant #RR08124-03. The aid of Dr. Melchor Ortiz, Jr., School of Public Health, University of Texas Health Sciences at Houston is gratefully acknowledged.Google Scholar