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Morphological, Immunohistochemical, and Ultrastructural Studies of the Donkey's Eye with Special Reference to the AFGF and ACE Expression

Part of: Micrographia Collection

Published online by Cambridge University Press:  06 May 2022

Abdelraheim H. Attaai Open the ORCID record for Abdelraheim H. Attaai [Opens in a new window] ,
Manal T. Hussein Open the ORCID record for Manal T. Hussein [Opens in a new window] ,
Khaled H. Aly  and
Fatma M. Abdel-Maksoud
Abdelraheim H. Attaai*
Affiliation:
Department of Anatomy and Embryology, Faculty of Veterinary Medicine, Assiut University, Assiut 71526, Egypt
Manal T. Hussein
Affiliation:
Department of cell and tissues, Faculty of Veterinary Medicine, Assiut University, Assiut 71526, Egypt
Khaled H. Aly
Affiliation:
Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, University of Bisha, Bisha 61922, P.O. Box 551, Saudi Arabia
Fatma M. Abdel-Maksoud
Affiliation:
Department of Anatomy and Embryology, Faculty of Veterinary Medicine, Assiut University, Assiut 71526, Egypt
*
*Corresponding author: Abdelraheim Attaai, E-mail: [email protected]
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Abstract

The donkey is mainly used as a working animal for riding and pack transport, as well as for dairy and meat production. Eye afflictions are common in donkeys, thus requiring a detailed study. A few studies had focused on the donkey's eye, and most of them had considered it, merely, a horse's eye. This study aimed to investigate the anatomy, histology, ultrastructure, and immunohistochemical features of the donkey's eye. The results were recorded and compared to those of horses in certain dimensions. Unlike horses, the donkey's eye is more circular in the contour of the cornea, has smaller lenticular thickness, and has longer anterior and vitreous chambers. Positive immunoreactivity to acidic fibroblast growth factor in the basal cell layers of the cornea was observed, indicating their role in cell differentiation and the renewal of the epithelium. Moreover, the corneal keratocytes expressed angiotensin-converting enzyme, which plays a role in corneal homeostasis and wound healing. Additionally, telocytes, hyalocytes, and other immune cells were observed within the iris and ciliary processes. Hence, this work is an updated detailed study of the morphology and ultrastructure of the donkey's eye and reveals some similarities and dissimilarities to the horse's eyes, which should be considered in clinical practice.

Keywords

ACEAFGFdonkey eyehyalocytesTCs
Type
Micrographia
Information
Microscopy and Microanalysis , Volume 28 , Issue 5 , October 2022 , pp. 1780 - 1793
Copyright
Copyright © The Author(s), 2022. Published by Cambridge University Press on behalf of the Microscopy Society of America

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