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Morphological Changes in the Motor Endplate and in the Belly Muscle Induced by Previous Static Stretching to the Climbing Protocol

Published online by Cambridge University Press:  23 July 2021

Gabriela K. Barbosa Open the ORCID record for Gabriela K. Barbosa [Opens in a new window] ,
Carolina dos S. Jacob Open the ORCID record for Carolina dos S. Jacob [Opens in a new window] ,
Mariana P. Rodrigues Open the ORCID record for Mariana P. Rodrigues [Opens in a new window] ,
Lara C. Rocha Open the ORCID record for Lara C. Rocha [Opens in a new window] ,
Jurandyr Pimentel Neto Open the ORCID record for Jurandyr Pimentel Neto [Opens in a new window]  and
Adriano P. Ciena Open the ORCID record for Adriano P. Ciena [Opens in a new window]
Gabriela K. Barbosa
Affiliation:
Department of Physical Activity, Laboratory of Morphology and Physical Activity – LAMAF, Institute of Biosciences (IB), São Paulo State University – UNESP, Rio Claro13506-900, SP, Brazil
Carolina dos S. Jacob
Affiliation:
Department of Physical Activity, Laboratory of Morphology and Physical Activity – LAMAF, Institute of Biosciences (IB), São Paulo State University – UNESP, Rio Claro13506-900, SP, Brazil
Mariana P. Rodrigues
Affiliation:
Department of Physical Activity, Laboratory of Morphology and Physical Activity – LAMAF, Institute of Biosciences (IB), São Paulo State University – UNESP, Rio Claro13506-900, SP, Brazil
Lara C. Rocha
Affiliation:
Department of Physical Activity, Laboratory of Morphology and Physical Activity – LAMAF, Institute of Biosciences (IB), São Paulo State University – UNESP, Rio Claro13506-900, SP, Brazil
Jurandyr Pimentel Neto
Affiliation:
Department of Physical Activity, Laboratory of Morphology and Physical Activity – LAMAF, Institute of Biosciences (IB), São Paulo State University – UNESP, Rio Claro13506-900, SP, Brazil
Adriano P. Ciena*
Affiliation:
Department of Physical Activity, Laboratory of Morphology and Physical Activity – LAMAF, Institute of Biosciences (IB), São Paulo State University – UNESP, Rio Claro13506-900, SP, Brazil
*
*Corresponding author: Adriano P. Ciena, E-mail: [email protected]
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Abstract

Static stretching provides benefits to the range of motion, modulates intramuscular connective tissue, and is incorporated into warm-up exercises. In this study, we present the effects in the motor endplate and belly muscle resulting from previous static stretching to climbing training. Twenty-four adult male Wistar rats were divided into four groups (n = 6 each): Sedentary (Sed), Climbing (Clb), Static stretching (Ss), and Static stretching prior to climbing (Ssc). The animals (Clb, Ss, and Ssc groups) were subjected to a training protocol 3×/week for 8 weeks, and the Ssc group was subjected to the Ss and Clb protocols in the same session. Samples from the animals were processed for immunostaining, histochemistry, and light microscopy. The Clb group presented a higher motor endplate; the Ss group presented no changes in the motor endplate; and the Ssc group demonstrated a higher compactness. We concluded that static stretching prior to the climbing protocol maintained the density of the motor endplate and increased the compactness of the neuromuscular junction structure. Also, there was a reduction in the myofibers’ diameter (Type I and IIa), an increase in myofibrillar densities (Type I and IIx, and total), and the reorganization of the myonuclei and the interstitium.

Keywords

cholinergic receptorsconnective tissuegastrocnemius muscleneuromuscular junctionskeletal muscle fibers
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 27 , Issue 5 , October 2021 , pp. 1183 - 1191
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Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press on behalf of the Microscopy Society of America

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