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Monitoring of Cell Surface Movement at the Erythrocyte’s Edge By Scanning Phase Contrast.Microscopy

Published online by Cambridge University Press:  02 July 2020

Arkady Bitler ,
Alexander Barbul  and
Rafi Korenstein
Arkady Bitler
Affiliation:
Department of Physiology and Pharmacology, Sackler Faculty of Medicine, Ramat Avi, 69978, Tel-Aviv, Israel. e-mail:[email protected]
Alexander Barbul
Affiliation:
Department of Physiology and Pharmacology, Sackler Faculty of Medicine, Ramat Avi, 69978, Tel-Aviv, Israel. e-mail:[email protected]
Rafi Korenstein
Affiliation:
Department of Physiology and Pharmacology, Sackler Faculty of Medicine, Ramat Avi, 69978, Tel-Aviv, Israel. e-mail:[email protected]
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Extract

We explored the ability of a novel method of nano-scale movement detection to analyze the effects of chemical crosslinking and thiol reduction on the fluctuations of the human erythrocyte membrane. The method employs line images of light intensity distribution across the red blood cell, obtained by laser scanning phase contrast microscope. The essential feature of these images is a noiseless region corresponding to edge scene. This enables- one to carry out very accurate fitting procedure between the theoretical intensity distribution and the experimental one. One of the free parameters of fitting is the edge position. Measuring line scan intensity distributions with a certain sampling rate and defining the edge positions in consequent moments of time, yields the time-dependent displacement of cell's edge with up to nanometer scale precision.

The significant feature of the suggested procedure is that it implicitly takes into account the halo effect.

Type
Novel Approaches to Microscopy Of Living Cells
Information
Microscopy and Microanalysis , Volume 5 , Issue S2: Proceedings: Microscopy & Microanalysis '99, Microscopy Society of America 57th Annual Meeting, Microbeam Analysis Society 33rd Annual Meeting, Portland, Oregon August 1-5, 1999 , August 1999 , pp. 1056 - 1057
Copyright
Copyright © Microscopy Society of America

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References

1.Bitler, A. et al. J. Microscop. (in-press).Google Scholar
2.Wang, Z. et al. Opt. Eng. 35 (1996) 2327CrossRefGoogle Scholar
3.Levin, S. and Korenstein, R.. Biophys. J. 60 (1991) 733CrossRefGoogle Scholar