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Modified Flat-BED Scanner for Macroscopic Study of Large Specimens

Published online by Cambridge University Press:  02 July 2020

Wayne Y. Cheng ,
Ping-chin Cheng ,
Mai-Ing Cheng  and
David B. Walden
Wayne Y. Cheng
Affiliation:
Williamsville East High School, Williamsville, NY, 14051, USA
Ping-chin Cheng
Affiliation:
Department of Electrical Engineering, University at Buffalo, Buffalo, NY, 14260USA
Mai-Ing Cheng
Affiliation:
AMIL Technologies, Williamsville, NY14221USA
David B. Walden
Affiliation:
Department of Plant Sciences, University of Western Ontario, London, Ontario, CanadaN6A 5B7
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Abstract

The field number of the objective lens limits the field of view in a conventional compound microscope; even at very low magnification, the microscope does not allow observation of large pieces of specimen at once. This made the observation of organs and large pieces of tissue sections inconvenient. in the course of studying the development of maize stem, we encountered the need of digitizing large hand sections for 3D reconstruction. Splicing a series of images that are taken at different positions to form a montage is not acceptable, because the jointed image suffers from mismatch due to lens distortion. Considering the resolution needed in our study, one alternative is to use a consumer flatbed scanner designed for a personal computer. in order to adapt the conventional scanner for wet tissue scanning, an Acer 620TPC flatbed scanner, equipped with a trans-illuminator, was modified (FIG 1).

Type
Student Research Forum (Organized by R. Koch and Z. Mason)
Information
Microscopy and Microanalysis , Volume 7 , Issue S2: Proceedings: Microscopy & Microanalysis 2001, Microscopy Society of America 59th Annual Meeting, Microbeam Analysis Society 35th Annual Meeting, Long Beach, California August 5-9, 2001 , August 2001 , pp. 458 - 459
Copyright
Copyright © Microscopy Society of America 2001

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