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Minimal Resin Embedding of Multicellular Specimens for Targeted FIB-SEM Imaging

Published online by Cambridge University Press:  04 August 2017

Nicole Schieber ,
Pedro Machado ,
Sebastian Markert ,
Christian Stigloher ,
Yannick Schwab  and
Anna Steyer
Nicole Schieber
Affiliation:
Cell Biology and Biophysics Unit, European Molecular Biology Laboratory, Heidelberg, Germany
Pedro Machado
Affiliation:
Electron Microscopy Core Facility, European Molecular Biology Laboratory, Heidelberg, Germany.
Sebastian Markert
Affiliation:
University of Wurzburg, Imaging Core Facility, Biocenter, Germany.
Christian Stigloher
Affiliation:
University of Wurzburg, Imaging Core Facility, Biocenter, Germany.
Yannick Schwab
Affiliation:
Cell Biology and Biophysics Unit, European Molecular Biology Laboratory, Heidelberg, Germany Electron Microscopy Core Facility, European Molecular Biology Laboratory, Heidelberg, Germany.
Anna Steyer
Affiliation:
Cell Biology and Biophysics Unit, European Molecular Biology Laboratory, Heidelberg, Germany

Abstract

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Information
Microscopy and Microanalysis , Volume 23 , Supplement S1: Proceedings of Microscopy & Microanalysis 2017 , July 2017 , pp. 1274 - 1275
Copyright
© Microscopy Society of America 2017 

References

[1] Kizilyaprak, C., et al FIB-SEM tomography in biology. Methods Mol Biol 2014 1117, p. 541–558.Google Scholar
[2] Belu, A., et al Ultra-thin resin embedding method for scanning electron microscopy of individual cells on high and low aspect ratio 3D nanostructures. J Microsc 2016 263(1p. 78–86.Google Scholar
[3] We would like to thank Sevi Durdu (Gilmour lab, EMBL Heidelberg) for providing the zebrafish. Darren Gilmour for giving valuable feedback to the project. Hernando Martinez Vergara (Arendt lab; EMBL Heidelberg) for providing the Platynereis and Detlev Arendt for his support in the project. We would like to thank the Electron Microscopy Core Facility (EMCF, EMBL Heidelberg) for their support.Google Scholar