Hostname: page-component-586b7cd67f-t7fkt Total loading time: 0 Render date: 2024-12-01T04:02:54.477Z Has data issue: false hasContentIssue false
  • English
  • Français

Microscopic Investigations of the Infection Process of Choristoneura Fumiferana Nucleopolyhedrovirus in the Spruce Budworm

Published online by Cambridge University Press:  02 July 2020

A. J. Brownwright ,
J. W. Barrett ,
T. R. Ladd ,
M. Primavera ,
S. S. Sohi ,
B. M. Arif ,
A. Retnakaran  and
S. R. Palli
A. J. Brownwright
Affiliation:
Great Lakes Forestry Centre, Canadian Forest Service, 1219 Queen St. East, P.O.Box 490, Sault Ste. Marie, OntarioP6A 5M7, Canada
J. W. Barrett
Affiliation:
Great Lakes Forestry Centre, Canadian Forest Service, 1219 Queen St. East, P.O.Box 490, Sault Ste. Marie, OntarioP6A 5M7, Canada
T. R. Ladd
Affiliation:
Great Lakes Forestry Centre, Canadian Forest Service, 1219 Queen St. East, P.O.Box 490, Sault Ste. Marie, OntarioP6A 5M7, Canada
M. Primavera
Affiliation:
Great Lakes Forestry Centre, Canadian Forest Service, 1219 Queen St. East, P.O.Box 490, Sault Ste. Marie, OntarioP6A 5M7, Canada
S. S. Sohi
Affiliation:
Great Lakes Forestry Centre, Canadian Forest Service, 1219 Queen St. East, P.O.Box 490, Sault Ste. Marie, OntarioP6A 5M7, Canada
B. M. Arif
Affiliation:
Great Lakes Forestry Centre, Canadian Forest Service, 1219 Queen St. East, P.O.Box 490, Sault Ste. Marie, OntarioP6A 5M7, Canada
A. Retnakaran
Affiliation:
Great Lakes Forestry Centre, Canadian Forest Service, 1219 Queen St. East, P.O.Box 490, Sault Ste. Marie, OntarioP6A 5M7, Canada
S. R. Palli
Affiliation:
Great Lakes Forestry Centre, Canadian Forest Service, 1219 Queen St. East, P.O.Box 490, Sault Ste. Marie, OntarioP6A 5M7, Canada
Get access

Extract

Choristoneura fumiferana nucleopolyhedrovirus(CfMNPV) is a baculovirus that infects the spruce budworm, Choristoneura fumiferana(Cf), and a few other related species. To study the infection process of this virus within the spruce budworm, we have constructed a recombinant virus (CfMNPV-GFP) expressing the green fluorescence protein(GFP) under the control of the polyhedrin promoter of Autographa californica nucleopolyhedrovirus. Each larva was fed 10,000 CfMNPV-GFP occlusion bodies(OB) applied to diet plugs and various tissues were dissected from the larvae at 24 hr intervals and examined for the presence of the OB and/or GFP using a fluorescence microscope. Larvae were also fixed and embedded for light and electron microscopy.

GFP-specific fluorescence was first detected as a few spots at the posterior end of the midgut at 72 hr post feeding(PF). By 96 hr PF, the fluorescence had expanded to include adjacent cells and some hemocytes.

Type
Detection and Application of Green (and other Colored) Fluorescent Proteins
Information
Microscopy and Microanalysis , Volume 4 , Issue S2: Proceedings: Microscopy & Microanalysis '98, Microscopy Society of America 56th Annual Meeting, Microbeam Analysis Society 32nd Annual Meeting, Atlanta, Georgia July 12-16, 1998 , July 1998 , pp. 1016 - 1017
Copyright
Copyright © Microscopy Society of America

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)