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The Lyme Disease Spirochete, Borrelia Burgdorferi, Forms Spheroplasts In the Presence of Spingolipid Analogue Ppmp

Published online by Cambridge University Press:  02 July 2020

Lori L. Lubke  and
Claude F. Garon
Lori L. Lubke
Affiliation:
Bacterial Pathogenesis Section, Rocky Mountain Laboratories Microscopy Branch, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana59840
Claude F. Garon
Affiliation:
Bacterial Pathogenesis Section, Rocky Mountain Laboratories Microscopy Branch, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana59840
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Lyme disease is the most common arthropod-borne infection in the United States. Over 100,000 cases have been reported to date. The disease is a multisystem disorder with dermatologic, neurologic and rheumatologic manifestations. The causative agent, Borrelia burgdorferi1has been isolated from ticks, animals and humans from around the world and can generally be cultured in the laboratory using BSK-II medium.

While biologically significant concentrations of eukaryotic cell inhibitors such as aphidicolin, ara C, cis platinum, CPX, hydroxyurea, mimosine, nalidixic acid, trioxsalen and boromethyglycine showed little effect on the growth of Borrelia burgdorferiwhen added to BSK-II cultures, PPMP, a spingolipid analogue, showed immediate and profound inhibitory effects when monitored by dark-field microscopy, transmission electron microscopy (TEM), field emission scanning electron microscopy (SEM) and by optical density measurements at 600 mμ. PPMP (dl-thzreo-l-phenyl-2-palmitoylamino-3-morpholino-l-propanol) is known to inhibit the synthesis of spingomyelin in Chinese hamster ovary cells and to inhibit the synthesis of glucosylceramides in a large variety of mammalian cells.

Type
Microbiology
Information
Microscopy and Microanalysis , Volume 5 , Issue S2: Proceedings: Microscopy & Microanalysis '99, Microscopy Society of America 57th Annual Meeting, Microbeam Analysis Society 33rd Annual Meeting, Portland, Oregon August 1-5, 1999 , August 1999 , pp. 1136 - 1137
Copyright
Copyright © Microscopy Society of America

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References

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