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Long-Term Ultrastructural Indices of Lead Intoxication in Pulmonary Tissue of the Rat

Published online by Cambridge University Press:  28 August 2013

Katarzyna Kaczyńska ,
Michał Walski  and
Małgorzata Szereda-Przestaszewska
Katarzyna Kaczyńska*
Affiliation:
Laboratory of Respiratory Reflexes, Polish Academy of Sciences Mossakowski Medical Research Centre, 02-106 Warsaw, 5 Pawińskiego Street, Poland
Michał Walski
Affiliation:
Laboratory of the Cell Ultrastructure, Polish Academy of Sciences Mossakowski Medical Research Centre, 02-106 Warsaw, 5 Pawińskiego Street, Poland
Małgorzata Szereda-Przestaszewska
Affiliation:
Laboratory of Respiratory Reflexes, Polish Academy of Sciences Mossakowski Medical Research Centre, 02-106 Warsaw, 5 Pawińskiego Street, Poland
*
*Corresponding author. E-mail: [email protected]
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Abstract

In the present research long-term pulmonary toxicity of lead was investigated in rats treated by intraperitoneal administration of lead acetate for three consecutive days (25 mg/kg per day). Five weeks after treatment average lead content in the whole blood was 0.41 μg/dL ± 0.05, in the lung homogenates it measured 3.35 μg/g ± 0.54, as compared to the control values of 0.13 ± 0.07 μg/dL and 1.03 μg/g ± 0.59, respectively. X-ray microanalysis of lung specimens displayed lead localized mainly within type II pneumocytes and macrophages. At the ultrastructural level the effects of lead toxicity were found in lung capillaries, interstitium, epithelial cells, and alveolar lining. Alveolar septa showed intense fibrosis, consisting of collagen, elastin, and fibroblasts. Thinned alveolar septa had emphysematous tissue with some revealing signs of angiogenesis. Type II pneumocytes contained lamellar bodies with features of laminar destruction. Fragments of the surfactant layer were often detached from the alveolar epithelium. These findings indicate that 5 weeks after exposure, lead provokes reconstruction of the alveolar septa including fibrosis and emphysematous changes in the lung tissue.

Keywords

lead toxicityelectron microscopylung parenchymafibrosisrat
Type
Biomedical and Biological Applications
Information
Microscopy and Microanalysis , Volume 19 , Issue 6 , December 2013 , pp. 1410 - 1415
Copyright
Copyright © Microscopy Society of America 2013 

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References

Adamson, I.Y.R., Prieditis, H., Hedgecock, C. & Vincent, R. (2000). Zinc is the toxic factor in the lung response to an atmospheric particulate sample. Toxicol Appl Pharmacol 166, 111–119.Google Scholar
Akers, I.A., Parsons, M., Hill, M.R., Hollenberg, M.D., Sanjar, S., Laurent, G.J. & McAnulty, R.J. (2000). Mast cell tryptase stimulates human lung fibroblast proliferation via protease-activated receptor-2. Am J Physiol Lung Cell Mol Physiol 278, L193–L201.Google Scholar
Alcaraz-Contreras, Y., Garza-Ocanas, L., Carcano-Diaz, K. & Ramirez-Gomez, X.S. (2011). Effect of glycine on lead mobilization, lead-induced oxidative stress, and hepatic toxicity in rats. J Toxicol 2011, 430539-1–7.Google Scholar
Bakshi, M.S., Zhao, L., Smith, R., Possmayer, F. & Petersen, N.O. (2008). Metal nanoparticle pollutants interfere with pulmonary surfactant function in vitro . Biophysic J 94, 855–868.Google Scholar
Bazzy, P., Bargahi, A., Zare, M., Moshtaghi, D., Movahhednia, M. & Akbarzade, S. (2012). Fibrotic changes of renal tissue following long exposure of lead acetate in male rabbits. Res Pharm Sci 7, S146. Google Scholar
Brown, R.W. & Longoria, T. (2010). Multiple risk factors for lead poisoning in hispanic sub-populations: A review. J Immigrant Minority Health 12, 715–725.Google Scholar
Carmeliet, P. (2003). Angiogenesis in health and disease. Nat Med 9, 653–659.CrossRefGoogle ScholarPubMed
Daston, G.P. (1981). Effects of cadmium on the prenatal ultrastructural maturation of rat alveolar epithelium. Teratology 23, 75–84.Google Scholar
Donelly, L.E. & Barnes, P.J. (2012). Defective phagocytosis in airways disease. Chest 141, 1055–1062.Google Scholar
Fortoul, T.I., Moncada-Hernandez, S., Saldivar-Osorio, L., Espejel-Maya, G., Mussali-Galante, P., del Carmen Avila-Casado, M., Colín-Barenque, L., Hernández-Serrato, M.I. & Avila-Costa, M.R. (2005). Sex differences in bronchiolar epithelium response after the inhalation of lead acetate (Pb). Toxicology 207, 323–330.CrossRefGoogle ScholarPubMed
Griffin, T.B., Coulston, F., Wills, H. & Russell, J.C. (1975). Biologic effects of airborne particulate lead on continuously exposed rats and rhesus monkeys. Environ Qual Saf Suppl 2, 202–220.Google Scholar
Gwaltney-Brant, S.M. (2002). Heavy metals. In Handbook of Toxicologic Pathology I, Haschek, W.M., Rousseaux, C.G. & Wallig, M.A. (Eds.), pp. 712–716. San Diego, San Francisco, New York, Boston, Sydney, Tokyo: Academic Press.Google Scholar
Jarup, L. (2003). Hazards of heavy metal contamination. Br Med Bull 68, 167–182.CrossRefGoogle ScholarPubMed
Kaczyńska, K., Walski, M. & Szereda-Przestaszewska, M. (2011). Ultrastructural changes in lung tissue after acute lead intoxication in the rat. J Electron Microsc (Tokyo) 60, 289–294.Google Scholar
Kotsariev, O.S., Antoniuk, S.V. & Lykholat, O.A. (2001). Structural-functional characteristics of the air-blood barrier of lungs upon inhalation of low concentrations of lead salt. Fiziol Zh 47, 36–41.Google Scholar
Martinez, F.O., Helming, L. & Gordon, S. (2009). Alternative activation of macrophages: An immunologic functional perspective. Annu Rev Immunol 27, 451–483.Google Scholar
Peter, F. & Strunc, G. (1983). Effect of ingested lead on concentration of blood and tissue lead in rabbits. Clin Biochem 16, 202–205.Google Scholar
Prozialeck, W.C., Edwards, J.R., Nebert, D.W., Woods, J.M., Barchowsky, A. & Atchison, W.D. (2008). The vascular system as a target of metal toxicity. Toxicol Sci 102, 201–218.Google Scholar
Sansar, W., Ahboucha, S. & Gamrani, H. (2011). Chronic lead intoxication affects glial and neural systems and induces hypoactivity in adult rat. Acta Histochem 113, 601–607.Google Scholar
Sansar, W., Bouyatas, M.M., Ahboucha, S. & Gamrani, H. (2012). Effects of chronic lead intoxication on rat serotoninergic system and anxiety behaviour. Acta Histochem 114, 41–45.Google Scholar
Sharma, V. & Pandey, D. (2010). Protective role of Tinspora cordifolia against lead-induced hepatotoxicity. Toxicol Int 17, 12–17.CrossRefGoogle Scholar
Strużyńska, L., Bubko, I., Walski, M. & Rafalowska, U. (2001). Astroglial reaction during the early phase of acute lead toxicity in the adult rat brain. Toxicology 165, 121–131.Google Scholar
Sun, H.W., Ma, D.J., Chao, C.Y., Liu, S. & Yuan, Z.B. (2009). Lead distribution in blood and organs of mice exposed to lead by vein injection. Environ Technol 30, 1051–1057.Google Scholar
Takano, Y., Taguchi, T., Suzuki, I., Balis, J.U. & Yuri, K. (2002). Cytotoxicity of heavy metals on primary cultured alveolar type II cells. Environ Res 89, 138–145.Google Scholar
Tátrai, E., Náray, M., Brózik, M., Adamis, Z. & Ungváry, G. (1998). Combined pulmonary toxicity of diethyldithiocarbamate and lead (II) oxide in rats. J Appl Toxicol 18, 33–37.Google Scholar
Tylko, G., Karasinski, J., Wroblewski, R., Roomans, G.M. & Kilarski, W.M. (2000). Electron probe X-ray microanalysis of cultured myogenic c2c12 cells with scanning and scanning transmission electron microscopy. Folia Histochem Cytobiol 38, 79–84.Google Scholar
Whitsett, J.A., Wert, S.E. & Weaver, T.E. (2010). Alveolar surfactant homeostasis and the pathogenesis of pulmonary disease. Annu Rev Med 61, 105–119.Google Scholar
Zelikoff, J.T., Parsons, E. & Schlesinger, R.B. (1993). Inhalation of particulate lead oxide disrupts pulmonary macrophage-mediated functions important for host defense and tumor surveillance in the lung. Environ Res 62, 207–222.Google Scholar
Zhao, C.Z., Fang, X.C., Wang, D., Tang, F.D. & Wang, X.D. (2010). Involvement of type II pneumocytes in the pathogenesis of chronic obstructive pulmonary disease. Respir Med 104, 1391–1395.Google Scholar