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Localization Of Camkiiα mrnas to Dendritic Spines Visualized By High Resolution In Situ Hybridization

Published online by Cambridge University Press:  02 July 2020

A.L. Byrd ,
S.M. Shenoy ,
M. Martinez ,
M. Plociniak ,
H. L. Zhang ,
R.H. Singer  and
G. J. Bassell
A.L. Byrd
Affiliation:
department of Neuroscience, Rose F. Kennedy Center for Mental Retardation, Albert Einstein College of Medicine, Bronx, New York10461
S.M. Shenoy
Affiliation:
Department of Anatomy and Structural Biology, Albert Einstein College of Medicine
M. Martinez
Affiliation:
department of Neuroscience, Rose F. Kennedy Center for Mental Retardation, Albert Einstein College of Medicine, Bronx, New York10461
M. Plociniak
Affiliation:
department of Neuroscience, Rose F. Kennedy Center for Mental Retardation, Albert Einstein College of Medicine, Bronx, New York10461
H. L. Zhang
Affiliation:
department of Neuroscience, Rose F. Kennedy Center for Mental Retardation, Albert Einstein College of Medicine, Bronx, New York10461
R.H. Singer
Affiliation:
Department of Anatomy and Structural Biology, Albert Einstein College of Medicine
G. J. Bassell
Affiliation:
department of Neuroscience, Rose F. Kennedy Center for Mental Retardation, Albert Einstein College of Medicine, Bronx, New York10461
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Extract

Observations that polyribosomes are localized near dendritic spines and beneath synapses has led to a hypothesis of synapse-specific gene expression in which local synthesis would provide a mechanism to influence synaptic structure and strength (l).The active transport of specific mRNAs into dendrites and spines may be a regulated mechanism to target synaptic and regulatory proteins to postsynaptic locations and influence synaptic activity. RNA granules labeled with the vital dye, SYT014, were observed to localize into developing neurites in response to the neurotrophin, NT-3 (2). Neurotrophins have been shown to enhance synaptic activity by a process which requires new protein synthesis (3). The identity and source of newly synthesized proteins which are required to enhance synaptic strength in response to NT-3 are unknown. CaMKIIαRNA localization into dendrites has been shown to occur during long term potentiation (4).

Type
Structural Approaches to the Study of Cell Cell Interactions In Three Dimensions
Information
Microscopy and Microanalysis , Volume 5 , Issue S2: Proceedings: Microscopy & Microanalysis '99, Microscopy Society of America 57th Annual Meeting, Microbeam Analysis Society 33rd Annual Meeting, Portland, Oregon August 1-5, 1999 , August 1999 , pp. 1030 - 1031
Copyright
Copyright © Microscopy Society of America

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References

1.Steward, O. et al., J. Neurocytology 25 (1996) 717.CrossRefGoogle Scholar
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