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Loading-Rate Dependent Cell Injury: A Design Criterion for Engineered Tissue Constructs

Published online by Cambridge University Press:  02 July 2020

Kenneth A. Barbee
Kenneth A. Barbee*
Affiliation:
School of Biomedical Engineering, Science, and Health Systems Drexel University, Philadelphia, PA19104
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Extract

Vascular endothelial cells experience a wide range of mechanical stimuli in vivo, including dynamic stretching and time-varying shear stress due to pulsatile blood flow. Under physiologic conditions, these hemodynamic forces constitute an important component of the cells' environment and are necessary for the normal maintenance of the structure and function of the vessel wall. Implantation of artificial vascular graft materials can significantly alter the mechanical environment of the cell. Furthermore, an important design criterion for engineered tissue constructs is the production of a mechanical environment that, ideally, stimulates normal growth and structural adaptation and. At least, avoids extremes in mechanical loading that might cause cellular trauma. In this paper, we investigate the mechanical loading conditions associated with normal and pathological mechanotransduction and cellular injury with an emphasis on the loading rate dependence of the cellular responses.

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Type
Biomaterials
Information
Microscopy and Microanalysis , Volume 6 , Issue S2: Proceedings: Microscopy & Microanalysis 2000, Microscopy Society of America 58th Annual Meeting, Microbeam Analysis Society 34th Annual Meeting, Microscopical Society of Canada/Societe de Microscopie de Canada 27th Annual Meeting, Philadelphia, Pennsylvania August 13-17, 2000 , August 2000 , pp. 984 - 985
Copyright
Copyright © Microscopy Society of America

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References

1. Blackman, BR, Barbee, KA, and Thibault., LE “In Vitro Cell Shearing Device to Investigate the Dynamic Response of Cells in a Controlled Hydrodynamic Environment” Ann Biomed Eng, 28: 1-11, 2000.CrossRefGoogle Scholar

2. Barbee, KA, Mundel, T, R Lai, , and Davies, PF. “Sub-Cellular Distribution of Shear Stress at the Surface of Flow-Aligned and Nonaligned Endothelial Monolayers.”. Am J Physiol 268(Heart Circ. Physiol. 37): 1765–1772 (1995).Google Scholar

3. Davies, PF, Mundel, T, and Barbee, KA. “A Mechanism for Heterogeneous Endothelial Responses to Flow In Vivo and In Vitro.” J Biomech 28(12):1553–1560 (1995).CrossRefGoogle ScholarPubMed

4. This work was supported by the CDC and the Allegheny-Singer Research Institute.Google Scholar