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Live Confocal Analysis of Fertilization and Early Development

Published online by Cambridge University Press:  02 July 2020

Uyen Tram  and
William Sullivan
Uyen Tram
Affiliation:
Department of Biology, Sinsheimer Labs, University of California, Santa Cruz, CA, 95064
William Sullivan
Affiliation:
Department of Biology, Sinsheimer Labs, University of California, Santa Cruz, CA, 95064
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Abstract

Embryonic development is a dynamic event and is best studied in live animals in real time. Much of our knowledge of the early events of embryogenesis, however, comes from immunofluourescent analysis of fixed embryos. While these studies provide an enormous amount of information about the organization of different structures during development, they can give only a static glimpse of a very dynamic event. More recently real-time fluorescent studies of living embryos have become much more routine and have given new insights to how different structures and organelles (chromosomes, centrosomes, cytoskeleton, etc.) are coordinately regulated. This is in large part due to the development of commercially available fluorescent probes, GFP technology, and newly developed sensitive fluorescent microscopes. For example, live confocal fluorescent analysis proved essential in determining the primary defect in mutations that disrupt early nuclear divisions in Drosophila melanogaster. For organisms in which GPF transgenics is not available, fluorescent probes that label DNA, microtubules, and actin are available for microinjection.

Type
Challenges of Confocal Microscopy in the 21st Century (Organized by S. Paddock)
Information
Microscopy and Microanalysis , Volume 7 , Issue S2: Proceedings: Microscopy & Microanalysis 2001, Microscopy Society of America 59th Annual Meeting, Microbeam Analysis Society 35th Annual Meeting, Long Beach, California August 5-9, 2001 , August 2001 , pp. 1012 - 1013
Copyright
Copyright © Microscopy Society of America 2001

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