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Light Microscopy, Confocal Laser Scanning Microscopy And Scanning And Transmission Electron Microscopy Of Cerebellar Golgi Cells.

Published online by Cambridge University Press:  02 July 2020

O. Castejόn  and
P Sims
O. Castejόn
Affiliation:
Instilulo de Invesligacioncs Biolόgicas, Facultad de Medicina, Univcrsidad del Zulia, Maracaibo, Venezuela
P Sims
Affiliation:
Biological and Biomatcrial Preparation Imaging and Characterization Laboratory, Animal Health and Biomedical Sciences, University of Wisconsin, Madison.
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Extract

The cerebellar cortex of albino mice, hamsters, teleost fishes, primates and human have been examined by correlative microscopy to study the Golgi cell soma, dendritic processes, axonal plexus and synaptic connections in the granular and molecular layers. For light microscopy (LM) toluidinc blue stained-plastic embedded scmithin sections and Golgi light microscopy preparations were used. For confocal laser scanning microscopy (CLSM) of hamster cerebellum the FM4-64 fluorescent stain was used as intracellular tracer (1). Conventional and high resolution scanning electron microscopy (SEM) of teleost fishes, primates and human were coated with gold-palladium and chromium (2). I Transmission electron microscopy (TEM). either by ullrathin sections or frccze-clching replicas, were examined to characterize synaptic connections in the granular and molecular layers. The Golgi cells appeared in the granular layer as polygonal, stellate, round or fusiform microncurons. 10-25 μm in maximal dimension, surrounded by the granule cell groups. Golgi light microscopy.

Type
Neurobiology
Information
Microscopy and Microanalysis , Volume 5 , Issue S2: Proceedings: Microscopy & Microanalysis '99, Microscopy Society of America 57th Annual Meeting, Microbeam Analysis Society 33rd Annual Meeting, Portland, Oregon August 1-5, 1999 , August 1999 , pp. 1302 - 1303
Copyright
Copyright © Microscopy Society of America

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References

1Castejόn, O.J and Sims, P.: Confocal laser scanning microscopy and scanning electron microscopy of cerebellar glomeruli Scanning 20 (1998) 199–200.Google Scholar
2Castejόn, O.J and Apkarian, RP. Conventional and high resolution scanning electron microscopy of outer and inner surface features of cerebellar nerve cells. J. Submicr. Cytol. Pathol 24 (1992). 549–562.Google Scholar
3Castejόn, O.J : Frcczc-fracture scanning electron microscopy and comparative freczc-ctching study of parallel fibcrPiirkinje spine synapses of vertebrate cerebellar cortex. J. Submicr. Cytol. Pathol. 22 (1990). 281–295.Google Scholar