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Light and Scanning Electron Microscopy, Energy Dispersive X-Ray Spectroscopy, and Histochemistry of Eucalyptus tereticornis

Part of: Micrographia Collection

Published online by Cambridge University Press:  09 August 2021

Priscilla S. de Brito ,
Carolina Sabedotti ,
Thiago B. Flores ,
Vijayasankar Raman ,
Julia E. Bussade ,
Paulo V. Farago  and
Jane Manfron
Priscilla S. de Brito
Affiliation:
Programa de Pós-graduação em Ciências Farmacêuticas, Universidade Estadual de Ponta Grossa, Carlos Cavalcanti Av., 4748, Ponta Grossa, PR 84030-900, Brazil
Carolina Sabedotti
Affiliation:
Graduação de Bacharelado em Farmácia, Universidade Estadual de Ponta Grossa, Ponta Grossa, PR 84030-900, Brazil
Thiago B. Flores
Affiliation:
Programa de Pós-graduação em Biologia Vegetal, Universidade Estadual de Campinas, São Paulo, SP, Brazil
Vijayasankar Raman
Affiliation:
National Center for Natural Products Research, School of Pharmacy, University of Mississippi, University, MS 38677, USA
Julia E. Bussade
Affiliation:
Department of Modern Languages, University of Mississippi, University, MS 38677, USA
Paulo V. Farago
Affiliation:
Programa de Pós-graduação em Ciências Farmacêuticas, Universidade Estadual de Ponta Grossa, Carlos Cavalcanti Av., 4748, Ponta Grossa, PR 84030-900, Brazil
Jane Manfron*
Affiliation:
Programa de Pós-graduação em Ciências Farmacêuticas, Universidade Estadual de Ponta Grossa, Carlos Cavalcanti Av., 4748, Ponta Grossa, PR 84030-900, Brazil
*
*Corresponding author: Jane Manfron, E-mail: [email protected]
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Abstract

This paper provides detailed anatomy and histochemistry of the leaves and stems of Eucalyptus tereticornis illustrated with brightfield and scanning electron microscopy. The key microscopic features that can aid in the species identification include the presence of crust-like epicuticular waxes on the leaf surfaces, platy aggregations of cluster crystals in the epidermal cells, presence of prismatic crystals in the epidermal cells, in the parenchymatous sheath in the blade and petiole, and in the cortex and pith of the stems, and leaf homogenous mesophyll consisting of palisade cells. Histochemical analyses confirmed the presence of lipophilic and phenolic compounds in the contents of secretory cavities, starch grains in the xylem parenchyma of the stem, and lignified elements in the sclerenchymatous ring adjoining the phloem and in the xylem in the leaves and stems.

Keywords

anatomyessential oilmicroscopyMyrtaceaesecretory cavity
Type
Micrographia
Information
Microscopy and Microanalysis , Volume 27 , Issue 5 , October 2021 , pp. 1295 - 1303
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Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press on behalf of the Microscopy Society of America

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