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Light and Electron Microscope Immunohistochemical and Biochemical Studies of Vitronectin in Developing Rat Bone.

Published online by Cambridge University Press:  02 July 2020

M. Takagi ,
T. Kumagai ,
I. Lee ,
Y. Ono  and
M. Maeno
M. Takagi
Affiliation:
Departments of Anatomy and 1-8-13 Kanda- Surugadai, Chiyoda-ku, Tokyo101, Japan
T. Kumagai
Affiliation:
Departments of Anatomy and 1-8-13 Kanda- Surugadai, Chiyoda-ku, Tokyo101, Japan
I. Lee
Affiliation:
Departments of Anatomy and 1-8-13 Kanda- Surugadai, Chiyoda-ku, Tokyo101, Japan
Y. Ono
Affiliation:
Departments of Anatomy and 1-8-13 Kanda- Surugadai, Chiyoda-ku, Tokyo101, Japan
M. Maeno
Affiliation:
Biochemistry, Nihon University School of Dentistry, 1-8-13 Kanda- Surugadai, Chiyoda-ku, Tokyo 101, Japan
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Extract

Recently, Seiffert using light microscope (LM) immunohistochemical methods, has localized vitronectin (Vn) in the mineralized bone matrix of mature mouse long bone after demineralization with 10 % nitric acid, indicating that Vn is a specific component of bone tissue. This raises the possibility that Vn is involved in regulation of bone metabolism. The present study extends previous studies to the electron microscope (EM) level and utilizes biochemical methods to determine the distribution and nature of Vn in early bone formation of developing rat mandible with rabbit antimurine Vn IgG (antiVn).

Developing jaws of fetuses were collected at embryonic day 15-18 from pregnant Wistar rats. After al-dehyde fixation, specimens with and without osmium post-fixation were dehydrated, and embedded in paraffin, Spurr's resin, or LR gold resin for morphological and immunohistochemical observations. Sections cut from paraffin- or LR gold resin-embedded specimens were immunostained with antiVn, which was kindly provided by Dr. D. Seiffert, The Scripps Research Institute, La Jolla, California.

Type
Developmental and Reproductive Biology
Information
Microscopy and Microanalysis , Volume 3 , Issue S2: Proceedings: Microscopy & Microanalysis '97, Microscopy Society of America 55th Annual Meeting, Microbeam Analysis Society 31st Annual Meeting, Histochemical Society 48th Annual Meeting, Cleveland, Ohio, August 10-14, 1997 , August 1997 , pp. 195 - 196
Copyright
Copyright © Microscopy Society of America 1997

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References

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