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Left-Handed Z-Rna In Lens Epithelium: Preequatorial Zone

Published online by Cambridge University Press:  02 July 2020

C.E. Gagna ,
H.R. Kuo  and
W.C. Lambert
C.E. Gagna
Affiliation:
Department of Pathology, UMDNJ-Medical School, Newark, NJ07103, USA
H.R. Kuo
Affiliation:
Department of Pathology, UMDNJ-Medical School, Newark, NJ07103, USA
W.C. Lambert
Affiliation:
Department of Pathology, UMDNJ-Medical School, Newark, NJ07103, USA
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Extract

Our goal was to determine the cellular localization of left-handed Z-RNA, within preeguatorial zone (PZ) epithelium of the normal adult dog ocular lens (1.5 yr) (Fig. 1), employing anti-Z-RNA IgG polyclonal antibodies. B-DNA has the ability to adopt the Z-DNA configuration in vitro(1). A-RNA can be transformed into Z-RNA under certain conditions (2). Z-RNA has been localized in cultured cells (3). Strong evidence supports the presence of Z-DNA in vivo (1). Elimination of DNA binding proteins by certain fixatives can initiate DNA supercoiling which stabilizes Z-DNA sequences (1). Z-DNA may play a role in regulating in vivo transcriptional enhancement (1).

Anti-Z-RNA antibody probes were produced in 3 rabbits immunized with injections of Z-RNA: Br-poly[ribosomal(G-C)]. Concerning light microscopy [immunohistochemistry (ABC method)], lens tissues were fixed in Carnoy's, embedded in paraffin and sectioned (3 μm) (Fig. 2). Image analysis was performed using a Leitz DM-RB microscope and Leica Quantiment 500 + image analyzer.

Type
Biological Microanalysis
Information
Microscopy and Microanalysis , Volume 5 , Issue S2: Proceedings: Microscopy & Microanalysis '99, Microscopy Society of America 57th Annual Meeting, Microbeam Analysis Society 33rd Annual Meeting, Portland, Oregon August 1-5, 1999 , August 1999 , pp. 1290 - 1291
Copyright
Copyright © Microscopy Society of America

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References

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