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Laser Scanning Confocal Microscopic Analysis of Cells Transfected with Genes of Varicella Zoster Virus

Published online by Cambridge University Press:  02 July 2020

Charles Grose ,
Christopher Hatfield ,
Julie Olson  and
Chantee Buranathai
Charles Grose
Affiliation:
Departments of Microbiology and Pediatrics, University of Iowa College of Medicine, Iowa City, IA52242.
Christopher Hatfield
Affiliation:
Departments of Microbiology and Pediatrics, University of Iowa College of Medicine, Iowa City, IA52242.
Julie Olson
Affiliation:
Departments of Microbiology and Pediatrics, University of Iowa College of Medicine, Iowa City, IA52242.
Chantee Buranathai
Affiliation:
Departments of Microbiology and Pediatrics, University of Iowa College of Medicine, Iowa City, IA52242.
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Extract

Varicella-zoster virus (VZV) is one of the human herpes viruses. It causes chickenpox in childhood; after chickenpox the virus remains in a latent state in the dorsal root ganglia of the spinal cord. When the virus reactivates in late adulthood, it causes the disease known as shingles or herpes zoster. The VZV genome includes approximately 70 open-reading frames, but only a few of the gene products have been well characterized.

The VZV glycoproteins include gH, gL, gE and gI. The gH:gL complex is an important viral fusogen. Through laser scanning confocal microscopy, we developed an assay to measure VZV-induced fusion and syncytial formation. VZV also encodes a cell surface Fc receptor, glycoprotein gE. VZV gE has previously been shown to display several features common to nonviral cell surface receptors. Attention focused on the ability of VZV gE to undergo receptor-mediated endocytosis. The transient transfection studies demonstrated by confocal microscopy and intemalization assays that VZV gE was endocytosed when expressed in HeLa cells. Endocytosis of gE was shown to be dependent on clathrin-coated vesicle formation within the cells.

Type
Solving Microbiological Problems With Microscopy
Information
Microscopy and Microanalysis , Volume 3 , Issue S2: Proceedings: Microscopy & Microanalysis '97, Microscopy Society of America 55th Annual Meeting, Microbeam Analysis Society 31st Annual Meeting, Histochemical Society 48th Annual Meeting, Cleveland, Ohio, August 10-14, 1997 , August 1997 , pp. 91 - 92
Copyright
Copyright © Microscopy Society of America 1997

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References

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