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Laser Capture Microdissection as an Aid to Ultrastructural Analysis

Published online by Cambridge University Press:  30 July 2002

Kenneth Grant
Affiliation:
Medical Center Blvd., Winston-Salem, NC 27157-1092, USA
W. Gray Jerome
Affiliation:
Medical Center Blvd., Winston-Salem, NC 27157-1092, USA Department of Pathology, Vanderbilt University Medical Center, B-4220 MCN, 1161 21st Ave., South, Nashville, TN 37232-2561, USA
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Abstract

Laser capture microdissection uses a microscope to identify specific cells for microdissection and then a laser-sensitive plastic to capture and remove the cells from their substrate. This efficient capture method was originally developed to capture cells for genetic analysis. However, it has also been used to capture cells for proteonomic analysis. In this article, we extend the uses of laser-capture microdissection by reporting a method for preparing captured cells for ultrastructural analysis by transmission electron microscopy. Cells prepared by our methodology show good fine structure preservation and are easily sectioned by standard ultramicrotomy.

Type
BIOLOGICAL APPLICATIONS
Copyright
2002 Microscopy Society of America

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