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Label-Free Characterization of Collagen Crosslinking in Bone-Engineered Materials Using Nonlinear Optical Microscopy

Published online by Cambridge University Press:  08 April 2021

Chao-Wei Hung
Affiliation:
PhD Program for Biomedical Engineering and Rehabilitation Science, China Medical University, No. 91, Hsueh-Shih Road, Taichung40402, Taiwan R.O.C.
Nirmal Mazumder
Affiliation:
Department of Biophysics, Manipal School of Life Sciences, Manipal Academy of Higher Education, Manipal, Karnataka576104, India
Dan-Jae Lin
Affiliation:
School of Dentistry, College of Dentistry, China Medical University, No. 91, Hsueh-Shih Road, Taichung40402, Taiwan R.O.C.
Wei-Liang Chen
Affiliation:
Center for Condensed Matter Sciences, National Taiwan University, Taipei10617, Taiwan R.O.C.
Shih-Ting Lin
Affiliation:
Integrative Stem Cell Center, China Medical University Hospital, No. 2, Yude Road, Taichung40447, Taiwan R.O.C.
Ming-Che Chan
Affiliation:
Institute of Photonic System, College of Photonics, National Chiao-Tung University, Tainan71150, Taiwan R.O.C. Institute of Biophotonics, National Yang-Ming University, No. 155, Sec. 2, Linong Street, Beitou District, Taipei City112, Taiwan R.O.C.
Guan-Yu Zhuo*
Affiliation:
Integrative Stem Cell Center, China Medical University Hospital, No. 2, Yude Road, Taichung40447, Taiwan R.O.C. Institute of New Drug Development, China Medical University, No. 91, Hsueh-Shih Road, Taichung40402, Taiwan R.O.C.
*
*Author for correspondence: Guan-Yu Zhuo, E-mail: [email protected]
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Abstract

Engineered biomaterials provide unique functions to overcome the bottlenecks seen in biomedicine. Hence, a technique for rapid and routine tests of collagen is required, in which the test items commonly include molecular weight, crosslinking degree, purity, and sterilization induced structural change. Among them, the crosslinking degree mainly influences collagen properties. In this study, second harmonic generation (SHG) and coherent anti-Stokes Raman scattering (CARS) microscopy are used in combination to explore the collagen structure at molecular and macromolecular scales. These measured parameters are applied for the classification and quantification among the different collagen scaffolds, which were verified by other conventional methods. It is demonstrated that the crosslinking status can be analyzed from SHG images and presented as the coherency of collagen organization that is correlated with the mechanical properties. Also, the comparative analyses of SHG signal and relative CARS signal of amide III band at 1,240 cm−1 to δCH2 band at 1,450 cm−1 of these samples provide information regarding the variation of the molecular structure during a crosslinking process, thus serving as nonlinear optical signatures to indicate a successful crosslinking.

Type
Biological Applications
Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press on behalf of the Microscopy Society of America

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Footnotes

Chao-Wei Hung and Nirmal Mazumder equally contributed to the present study.

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